A MICROWELL CYTOTOXICITY ASSAY USING ARTEMIA-SALINA (BRINE SHRIMP)
A MICROWELL CYTOTOXICITY ASSAY USING ARTEMIA-SALINA (BRINE SHRIMP)
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DOI：
10.1055/s-2006-959661
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发表时间：
1993-06-01
期刊：
影响因子：
2.7
通讯作者：
PHILLIPSON, JD
中科院分区：
文献类型：
--
作者：
SOLIS, PN;WRIGHT, CW;PHILLIPSON, JD
一种新的细胞毒性试验方法。已经开发了Salina，并显示出与先前公开的试管方法相当的结果。该试验可靠地检测到所有的化合物毒性KB细胞在一系列的21个抗疟原虫活性剂，除了两个需要代谢活化在man.Four quassinoids与细胞毒性和抗疟原虫活性也有毒的卤虫虾，而quassin本身是无活性的，在所有三个系统。有人提出，该测定提供了一种方便的手段，通过该手段可以检测植物提取物的分级分离过程中细胞毒性苦木素的存在。
A new microplate assay for cytotoxicity testing using A. salina has been developed and shown to give results comparable to a previously published test-tube method. The assay reliably detected all of the compounds toxic to KB cells in a series of 21 pharmacologically active agents, except for two which require metabolic activation in man. Four quassinoids with cytotoxic and antiplasmodial activity were also toxic to the brine shrimp while quassin itself was inactive in all three systems. It is proposed that this assay provides a convenient means by which the presence of cytotoxic quassinoids may be detected during the fractionation of plant extracts.