A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis.
A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis.
复制标题
SUMO依赖性蛋白网络调节卵母细胞减数分裂期间的染色体会议。
DOI：
10.1016/j.molcel.2016.11.001
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发表时间：
2017-01-05
期刊：
影响因子：
16
通讯作者：
Hay RT
中科院分区：
文献类型：
--
作者：
Pelisch F;Tammsalu T;Wang B;Jaffray EG;Gartner A;Hay RT
在秀丽隐杆线虫卵母细胞减数分裂过程中，位于同源染色体之间的多蛋白环复合物（RC）通过色动蛋白KLP-19的作用促进染色体的聚集。虽然一些RC组件是已知的，但RC组件的机制仍然不清楚。我们发现SUMO E3连接酶GEI-17/皮亚斯是KLP-19募集到RC所必需的，并且蛋白质组学分析鉴定KLP-19为体内SUMO底物。体外分析显示，KLP-19以GEI-17依赖性方式有效地被sumoylated，而GEI-17经历广泛的自sumoylation。GEI-17和另一个RC组分，激酶BUB-1，含有功能性SUMO相互作用基序（西姆斯），允许它们将SUMO修饰的蛋白质，包括KLP-19，招募到RC中。因此，动态SUMO修改和RC部件中西姆斯的存在产生了便于RC组装的SUMO-SIM网络。我们的研究结果突出了SUMO-SIM网络在调节动态蛋白质复合物组装中的重要性。SUMO偶联物在减数分裂过程中积累在中间二价体的环状复合物中。环状复合物的组装需要主动的SUMO化。SUMO E3连接酶GEI-17/皮亚斯在体内和体外将色动素KLP-19进行SUMO化。提供证据表明，高度动态的，协调的，空间约束的类小泛素化调节减数分裂过程中的染色体聚集在秀丽隐杆线虫卵母细胞。
During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localized between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC, and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO interaction motifs (SIMs), allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus, dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes. SUMO conjugates accumulate in the ring complex within the midbivalent during meiosis Ring complex assembly requires active sumoylation The SUMO E3 ligase GEI-17/PIAS sumoylates the chromokinesin KLP-19 in vivo and in vitro Meiotic chromosome congression depends on sumoylation and non-covalent SUMO binding Pelisch et al. provide evidence that highly dynamic, coordinated, and spatially constrained sumoylation regulates chromosome congression during meiosis in C. elegans oocytes.