Lipase oriented-immobilized on dendrimer-coated magnetic multi-walled carbon nanotubes toward catalyzing biodiesel production from waste vegetable oil
Lipase oriented-immobilized on dendrimer-coated magnetic multi-walled carbon nanotubes toward catalyzing biodiesel production from waste vegetable oil
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DOI：
10.1016/j.fuel.2016.03.071
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发表时间：
2016-08-15
期刊：
影响因子：
7.4
通讯作者：
Yan, Yunjun
中科院分区：
文献类型：
--
作者：
Fan, Yanli;Wu, Guiying;Yan, Yunjun
将聚酰胺-胺（PAMAM）树枝状大分子接枝到磁性多壁碳纳米管（mMWCNTs）上，使其具有联合收割机的磁性和大面积的氨基功能化表面。基于酶的三维结构分析，将米黑根毛霉脂肪酶（RML）定向固定在m-MWCNTs-PAMAM基质上。固定化脂肪酶的活力回收率达到2808%，其酯化活性是游离酶的27倍。在叔丁醇溶剂体系中，利用固定化酶催化废植物油制备生物柴油。在最佳条件下生物转化率可达94%。固定化脂肪酶易于回收，重复使用10次后转化率没有明显下降。结果表明，固定化RML是一种潜在的催化剂，具有高稳定性和良好的操作重复使用性的生物柴油生产。(C)2016爱思唯尔有限公司版权所有。
A polyamidoamine (PAMAM) dendrimer was grafted onto magnetic multi-walled carbon nanotubes (mMWCNTs) to combine magnetic properties with a large surface functionalized with amino groups. Based on three dimensional structural (3D) analysis of enzyme, oriented-immobilization of Rhizomucor miehei lipase (RML) on the obtained m-MWCNTs-PAMAM matrix was achieved. The recovery activity of the immobilized lipase was up to 2808% and the corresponding esterification activity was 27-fold higher than that of the free enzyme. The immobilized enzyme was employed to catalyze biodiesel production from waste vegetable oil in a tert-butanol solvent system. Biodiesel conversion reached 94% under optimal conditions. Moreover, the immobilized lipase could be easily recovered and there was no significant decrease in conversion rates after 10 cycles of reuse. The results suggested that the immobilized RML is a potential catalyst with high stability and excellent operational reusability for biodiesel production. (C) 2016 Elsevier Ltd. All rights reserved.