Legionella species diversity and dynamics from surface reservoir to tap water: from cold adaptation to thermophily
Legionella species diversity and dynamics from surface reservoir to tap water: from cold adaptation to thermophily
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DOI：
10.1038/ismej.2015.199
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发表时间：
2016-05-01
期刊：
影响因子：
11
通讯作者：
Hoefle, Manfred G.
中科院分区：
文献类型：
--
作者：
Lesnik, Rene;Brettar, Ingrid;Hoefle, Manfred G.
使用属特异性 PCR 扩增子和基于 16S rRNA 基因的单链构象多态性 (SSCP) 指纹分析，分析了布伦瑞克市饮用水供应系统 (DWSS) 的水样中的军团菌物种组成。这些分析涵盖了整个供应链，包括原水、处理过程和大规模储存，以及每月从冷热自来水中采样的成品饮用水的季节性研究。原水处理降低了军团菌的多样性和丰度，对军团菌产生了重大影响。自来水的军团菌物种组成与两种水源水的军团菌物种组成非常不同。在冷水中，每个样品观察到 8-14 种不同的军团菌 (PTL) 系统型，相对丰度范围为 >1% 至 53%。在热水中，嗜肺军团菌在所有季节都以较高的相对丰度（8-40%）存在，并伴有 5-14 个其他 PTL，其中 6 个 PTL 与冷水相同。这种嗜热军团菌群落，包括嗜肺军团菌，能够在 50 摄氏度以上的热水中生长。这种嗜热军团菌种群与饮用水管理和公共卫生具有普遍相关性，而且还与军团菌属的生态和进化相关。
Water samples of the Drinking Water Supply System (DWSS) of the city of Braunschweig were analysed for its Legionella species composition using genus-specific PCR amplicons and single-strand conformation polymorphism (SSCP) fingerprint analyses based on 16S rRNA genes. These analyses comprised the whole supply chain including raw water, treatment process and large-scale storage, and a seasonal study of finished drinking water sampled monthly from cold and hot tap water. Treatment of raw water had a major impact on Legionella species by reducing their diversity and abundances. The Legionella species composition of the tap water was highly distinct from that of both source waters. In cold water, 8-14 different phylotypes of Legionella (PTLs) were observed per sample with relative abundances ranging from >1% to 53%. In hot water, L. pneumophila was present during all seasons at high relative abundances (8-40%) accompanied by 5-14 other PTLs of which 6 PTLs were in common with cold water. This thermophilic Legionella community, including L. pneumophila, was able to grow in the hot water above 50 degrees C. Such thermophilic Legionella populations are of general relevance for drinking water management and public health, but also for the ecology and evolution of the genus Legionella.