DESTRUCTION OF THE CDC28 CLB MITOTIC KINASE IS NOT REQUIRED FOR THE METAPHASE TO ANAPHASE TRANSITION IN BUDDING YEAST
DESTRUCTION OF THE CDC28 CLB MITOTIC KINASE IS NOT REQUIRED FOR THE METAPHASE TO ANAPHASE TRANSITION IN BUDDING YEAST
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DOI：
10.1002/j.1460-2075.1993.tb05846.x
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发表时间：
1993-05-01
期刊：
影响因子：
11.4
通讯作者：
NASMYTH, K
中科院分区：
文献类型：
--
作者：
SURANA, U;AMON, A;NASMYTH, K
人们普遍认为，有丝分裂周期蛋白的降解导致有丝分裂cdc2/CDC28激酶活性降低，从而引发中期到后期的转变。对出芽酵母（Saccharomcyces cerevisiae）的两个观察结果与这种情况不一致：(i) cdc15突变体中存在高水平的激酶时，后期发生；（ii） b型有丝分裂周期蛋白的过量产生导致停滞，而不是像以前报道的那样在中期，而是在末期。因此，激酶的破坏与有丝分裂的退出有关，而不是进入后期。此外，esp1突变体的行为表明，在没有后期完成的情况下也会发生激酶破坏。因此，后期的执行和CDC28激酶活性的破坏似乎相互独立地发生。
It is widely assumed that degradation of mitotic cyclins causes a decrease in mitotic cdc2/CDC28 kinase activity and thereby triggers the metaphase to anaphase transition. Two observations made on the budding yeast Saccharomcyces cerevisiae are inconsistent with this scenario: (i) anaphase occurs in the presence of high levels of kinase in cdc15 mutants and (ii) overproduction of a B-type mitotic cyclin causes arrest not in metaphase as previously reported but in telophase. Kinase destruction is therefore implicated in the exit from mitosis rather than the entry into anaphase. The behaviour of esp1 mutants shows in addition that kinase destruction can occur in the absence of anaphase completion. The execution of anaphase and the destruction of CDC28 kinase activity therefore appear to take place independently of one another.