Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number.
Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number.
复制标题
利用 Wheat660K SNP 阵列衍生的高密度遗传图谱对籽粒数量的主要 QTL 进行高分辨率定位
DOI：
10.1038/s41598-017-04028-6
复制
发表时间：
2017-06-19
影响因子：
4.6
通讯作者：
Li JM
中科院分区：
文献类型：
--
作者：
Cui F;Zhang N;Fan XL;Zhang W;Zhao CH;Yang LJ;Pan RQ;Chen M;Han J;Zhao XQ;Ji J;Tong YP;Zhang HX;Jia JZ;Zhao GY;Li JM
在作物中，高密度的遗传连锁图谱对于遗传和基因组研究都是必不可少的。小麦基因组的复杂性和庞大性阻碍了高分辨率遗传图谱的获得。在这项研究中，我们报告了一个高密度的遗传图谱的基础上的个人作图群体使用的AffyesthetWheat660K单核苷酸多态性（SNP）阵列作为探针在六倍体小麦。该图谱包含119566个位点，总长度为4424.4cM，其中119001个位点为SNP标记。该遗传图谱与90 K和820 K的一致遗传图谱具有良好的共线性，与最近公布的小麦全基因组组装结果一致。高密度小麦遗传图谱将为今后小麦遗传和基因组研究提供重要资源。此外，基于高密度小麦遗传图谱对禾本科植物基因组进行了比较基因组学分析，对禾本科植物基因组间的结构关系进行了综述。本研究定位了一个稳定的穗粒数主效QTL，为进一步定位和图位克隆奠定了基础。
In crop plants, a high-density genetic linkage map is essential for both genetic and genomic researches. The complexity and the large size of wheat genome have hampered the acquisition of a high-resolution genetic map. In this study, we report a high-density genetic map based on an individual mapping population using the Affymetrix Wheat660K single-nucleotide polymorphism (SNP) array as a probe in hexaploid wheat. The resultant genetic map consisted of 119 566 loci spanning 4424.4 cM, and 119 001 of those loci were SNP markers. This genetic map showed good collinearity with the 90 K and 820 K consensus genetic maps and was also in accordance with the recently released wheat whole genome assembly. The high-density wheat genetic map will provide a major resource for future genetic and genomic research in wheat. Moreover, a comparative genomics analysis among gramineous plant genomes was conducted based on the high-density wheat genetic map, providing an overview of the structural relationships among theses gramineous plant genomes. A major stable quantitative trait locus (QTL) for kernel number per spike was characterized, providing a solid foundation for the future high-resolution mapping and map-based cloning of the targeted QTL.