Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): Comparison of genetic and serological data to characterise the EHDV serogroup
Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): Comparison of genetic and serological data to characterise the EHDV serogroup
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DOI：
10.1016/j.virusres.2009.07.012
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发表时间：
2009-11-01
期刊：
影响因子：
5
通讯作者：
Mertens, P. P. C.
中科院分区：
文献类型：
--
作者：
Anthony, S. J.;Maan, S.;Mertens, P. P. C.
流行性出血症病毒（EHDV）的外壳蛋白VP2和VP5在感染开始期间对宿主细胞结合是重要的。它们还已知用于确定病毒血清型。这项研究提出了一个完整的遗传和系统发育分析，这些蛋白质（和编码它们的基因），以允许比较选择压力作用于每个和相关的基因序列数据与血清型。登录号，基因和蛋白质的大小，ORF的位置，G+C含量，末端六核苷酸，起始和终止密码子和系统发育关系。结果表明，VP2是高度可变的，处于巨大的适应压力下，并且可以与血清型相关。虽然也是可变的，但VP5似乎比VP2承受更小的适应压力，但仍显示出与血清型的一些相关性。在这项研究中已经确定了七种EHDV血清型，尽管结果确实表明某些血清型是非常密切相关的，并强调了使用分子和血清学分析的好处。末端六核苷酸的分析表明，5'末端比3'末端处于更大的纯化选择下。也有证据表明，片段2和6（分别编码VP2和VP5）通过基因复制和随后的突变而生长。(C)2009 Elsevier B.V.保留所有权利。
The outer-coat proteins, VP2 and VP5, of epizootic haemorrhagic disease virus (EHDV) are important for host cell binding during the initiation of infection. They are also known to determine virus serotype. This study presents a complete genetic and phylogenetic analysis of these proteins (and the genes that code for them) to allow comparison of the selective pressures acting on each and the correlation of genetic sequence data with serotype. Accession numbers, gene and protein sizes, ORF positions, G+C contents, terminal hexanucleotides, start and stop codons and phylogenetic relationships are all presented. The results show that VP2 is highly variable, is under great pressure to adapt and can be correlated with serotype. While also variable, VP5 appears to be under less adaptive pressure than VP2 but still shows some correlation with serotype. Seven serotypes of EHDV have been defined in this study, although the results do show that some serotypes are extremely closely related-and highlight the benefit of using both molecular and serologic analyses. Analysis of the terminal hexanucleotides showed that the 5' terminus is under greater purifying selection than the 3'. Evidence is also presented that both segments 2 and 6 (coding for `VP2 and VP5 respectively) have grown via gene duplication and subsequent mutation. (C) 2009 Elsevier B.V. All rights reserved.