A novel method of mouse ex utero transplantation of hepatic progenitor cells into the fetal liver
A novel method of mouse ex utero transplantation of hepatic progenitor cells into the fetal liver
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DOI：
10.1016/j.bbrc.2009.02.037
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发表时间：
2009-04-03
影响因子：
3.1
通讯作者：
Teraoka, Hirobumi
中科院分区：
文献类型：
--
作者：
Shikanai, Mima;Asahina, Kinji;Teraoka, Hirobumi
避免成人肝脏生态位的限制，肝干/祖细胞移植到胎儿肝脏中是理想的，以分析在肝脏发育环境中的未成熟细胞。在此，我们建立了一种新的监测工具，用于监测通过子宫外手术移植到小鼠胎肝中的肝祖细胞的细胞命运。当将胚胎（艾德）14.5天的成肝细胞注射到ED 14.5胎肝中时，移植的细胞大量表达白蛋白或弱表达甲胎蛋白，并且在新生肝中含有糖原，表明移植的成肝细胞可以与周围的受体实质细胞一致地增殖和分化。移植的细胞在6周龄小鼠的肝脏中成熟。此外，该方法适用于小鼠胚胎干细胞来源的成肝细胞样细胞的移植。这些数据表明，这种独特的技术将提供一个新的体内实验系统，研究肝干/祖细胞的细胞命运和肝脏器官发生。(c)2009 Elsevier Inc. All rights reserved.
Avoiding the limitations of the adult liver niche, transplantation of hepatic stem/progenitor cells into fetal liver is desirable to analyze immature cells in a hepatic developmental environment. Here, we established a new monitor tool for cell fate of hepatic progenitor cells transplanted into the mouse fetal liver by using ex utero surgery. When embryonic day (ED) 14.5 hepatoblasts were injected into the ED14.5 fetal liver, the transplanted cells expressed albumin abundantly or alpha-fetoprotein weakly, and contained glycogen in the neonatal liver, indicating that transplanted hepatoblasts can proliferate and differentiate in concord with surrounding recipient parenchymal cells. The transplanted cells became mature in the liver of 6-week-old mice. Furthermore, this method was applicable to transplantation of hepatoblast-like cells derived from Mouse embryonic stem cells. These data indicate that this unique technique will provide a new in vivo experimental system for studying cell fate of hepatic stem/progenitor cells and liver organogenesis. (c) 2009 Elsevier Inc. All rights reserved.