Primary biodegradation and mineralization of aryl organophosphate flame retardants by Rhodococcus-Sphingopyxis consortium.
Primary biodegradation and mineralization of aryl organophosphate flame retardants by Rhodococcus-Sphingopyxis consortium.
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DOI：
10.1016/j.jhazmat.2021.125238
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发表时间：
2021-01
影响因子：
13.6
通讯作者：
Junhua Wang;T. S. Hlaing;M. T. Nwe;Mar Mar Aung-Mar;Chao Ren;Wei Wu;Yanchun Yan
中科院分区：
文献类型：
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作者：
Junhua Wang;T. S. Hlaing;M. T. Nwe;Mar Mar Aung-Mar;Chao Ren;Wei Wu;Yanchun Yan
本文研究了Rhodococcus-Sphingopyxisconsortium对芳基有机磷阻燃剂（aryl-OPFRs）的生物降解作用。YC-JH 2和Sphingopyxissp. YC-JH3.确定了两种复合菌株的最佳比例为1：1。在最适条件下（pH 8，35 °C和0%盐度），该菌能以芳基OPFRs为唯一碳源快速降解，对磷酸三苯酯（TPhP）、磷酸三甲苯酯（TCrP）和磷酸2-乙基己基二苯基酯（EHDPP）的半衰期分别为4.53，21.11和23.0 h。该菌群在pH（6-10）、温度（20-40 °C）和盐度（0-6%）范围内均保持较高的降解效率。该菌对高浓度的TPhP具有较好的降解能力，当浓度达到500 mg/L时，对TPhP的降解速率没有抑制作用。对金属离子和表面活性剂的影响进行了估计。除Zn ~（2+）和Pb ~（2+）对抑制作用无影响或有轻微促进作用外，其余金属离子均表现出明显的抑制作用。离子型表面活性剂会严重降低降解能力，而非离子型表面活性剂对降解能力无影响。在接种量充足的情况下，矿化度可在一周内达到75%以上。本研究为芳基OPFRs污染的生物修复提供了高效微生物。
In this study, the biodegradation towards aryl organophosphate flame retardants (aryl-OPFRs) was investigated by theRhodococcus-Sphingopyxisconsortium, mixture of strainRhodococcussp. YC-JH2 andSphingopyxissp. YC-JH3. The optimal ratio between the two composition strains was determined as 1:1. Under the optimum condition (pH 8, 35 °C and 0% salinity), the consortium could utilize aryl-OPFRs as sole carbon source and degrade them rapidly with half-life of 4.53, 21.11 and 23.0 h for triphenyl phosphate (TPhP), tricresyl phosphate (TCrP) and 2-ethylhexyl diphenyl phosphate (EHDPP) respectively. The consortium maintained high degrading efficiency under a wide of range of pH (6–10), temperature (20–40 °C) and salinity (0–6%). Besides, the consortium could rapidly degrade high concentration of TPhP and no inhibitory effect towards degradation speed was observed up to 500 mg/L. The effect of metal ions and surfactants was estimated. Most metal ions exhibited significant inhibition, except Zn2+and Pb2+, which showed no effect or slight promotion. Ionic surfactants could severely reduce the degrading capacity, while nonionic surfactants showed no effect. With abundant inoculation of the consortium, mineralization higher than 75% could be achieved within a week. This study provides efficient microorganisms for bioremediation of aryl-OPFRs contamination.