THE INTERACTION OF RB WITH E2F COINCIDES WITH AN INHIBITION OF THE TRANSCRIPTIONAL ACTIVITY OF E2F
THE INTERACTION OF RB WITH E2F COINCIDES WITH AN INHIBITION OF THE TRANSCRIPTIONAL ACTIVITY OF E2F
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DOI：
10.1101/gad.6.2.177
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发表时间：
1992-02-01
影响因子：
10.5
通讯作者：
NEVINS, JR
中科院分区：
文献类型：
--
作者：
HIEBERT, SW;CHELLAPPAN, SP;NEVINS, JR
最近的实验表明，E2F 转录因子与 PB1 基因产物形成复合物。 E2F-pRB 复合物可以使用从大肠杆菌中分离的 GST-RB 融合蛋白在体外测定中重建。这种相互作用取决于参与 E1A/T 抗原结合的 pRB 序列以及 E1A/T 结合不需要的羧基末端 pRB 序列。此外，重构分析表明，除了 E2F 和 pRB 之外，E2F-pRB 复合物的形成还需要辅助因子。转染实验中腺病毒E2启动子的转录分析表明，含有pRB和E2F的复合物的形成与E2F依赖性转录活性的抑制相一致。不与 E2F 结合的突变 pRB 蛋白不会抑制转录。我们得出结论，由于 pRB 与 E2F 相互作用，pRB 可能调节 E2F 因子的转录功能。
Recent experiments have shown that the E2F transcription factor is in a complex with the PB1 gene product. The E2F-pRB complex can be reconstituted in an in vitro assay using a GST-RB fusion protein isolated from Escherichia coli. This interaction is dependent on pRB sequences involved in E1A/T-antigen binding as well as carboxy-terminal pRB sequences that are not necessary for E1A/T binding. Moreover, reconstitution assays reveal a requirement for an accessory factor, in addition to E2F and pRB, for formation of the E2F-pRB complex. Assays of transcription from the adenovirus E2 promoter in transfection experiments demonstrate that formation of the complex containing pRB and E2F coincides with an inhibition of E2F-dependent transcriptional activity. A mutant pRB protein that does not associate with E2F does not inhibit transcription. We conclude that as a consequence of its interaction with E2F, pRB may regulate the transcriptional function of the E2F factor.