THE ISOFORMS OF C-PROTEIN AND THEIR DISTRIBUTION IN MAMMALIAN SKELETAL-MUSCLE
THE ISOFORMS OF C-PROTEIN AND THEIR DISTRIBUTION IN MAMMALIAN SKELETAL-MUSCLE
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DOI：
10.1007/bf00712585
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
PERRY, SV
中科院分区：
文献类型：
--
作者：
DHOOT, GK;HALES, MC;PERRY, SV
用人肌球蛋白粗品免疫小鼠，制备了特异性抗兔肌C蛋白慢骨骼肌亚型的单克隆抗体。它与兔骨骼肌的X蛋白组分反应，并染色该组织中的所有I型细胞。它还以不同的强度染色了一部分II型细胞。用慢C蛋白抗体染色的II型细胞也用针对兔快肌C蛋白制备的多克隆抗体染色。慢C蛋白抗体不染色的Ⅱ型细胞，快C蛋白抗体染色强烈。在人类骨骼肌中，慢C蛋白抗体染色所有细胞，而快C抗体仅染色II型细胞。它的结论是C蛋白在成年脊椎动物骨骼肌中的亚型的分布是更复杂的情况下，如肌钙蛋白复合物的组分的蛋白质。
A monoclonal antibody that is specific for the slow skeletal muscle isoform of C protein of rabbit muscle has been prepared by immunizing mice with a crude preparation of human myosin. It reacted with the X protein fraction of rabbit skeletal muscle and stained all type I cells in this tissue. It also stained a fraction of the type II cells with varying intensities. The type II cells staining with antibody to slow C protein also stained with a polyclonal antibody prepared against rabbit fast muscle C protein. The type II cells not staining with antibody to slow C protein stained strongly with antibody to fast C protein. In the human skeletal muscle antibody to slow C protein stained all cells whereas antibody to fast C stained only type II cells. It is concluded that the distribution of the isoforms of C protein in adult vertebrate skeletal muscle is more complex than is the case with proteins such as components of the troponin complex.