Reliability of synaptic transmission at the synapses of Held in vivo under acoustic stimulation.
Reliability of synaptic transmission at the synapses of Held in vivo under acoustic stimulation.
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DOI：
10.1371/journal.pone.0007014
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发表时间：
2009-10-02
期刊：
影响因子：
3.7
通讯作者：
Rübsamen R
中科院分区：
文献类型：
--
作者：
Englitz B;Tolnai S;Typlt M;Jost J;Rübsamen R
Held巨突触在高保真听觉处理中起着重要作用，并为中枢突触的突触传递提供了模型系统。最近的研究已经调查了动作电位的传递是否会在这些突触处失败。在耳蜗前腹侧核（AVCN）的Held的endbulbs出现了一致的图片，而在杯的Held的内侧核的斜方体（MNTB）的结果传输的可靠性仍然不一致。在体内，这种差异可能是由于难以识别传输失败。我们介绍了一种新的方法来检测不可靠的传输在体内。基于细胞波形和记录中的其他电位之间的时间关系，开发了一种统计测试，该测试提供了故障存在和不存在之间的平衡决策。它的性能量化使用模拟电压记录，并发现表现出高水平的准确性。该方法适用于细胞外记录从突触的举行在体内。在肾盏举行失败的传输被发现只有很少。相比之下，在endbulbs举行的AVCN故障被发现在自发的，兴奋的，和抑制的条件下。根据以前的研究，失败发生在最丰富的抑制条件下，这表明抑制的作用。在调查的活动条件/麻醉下，传输似乎在很大程度上保持畅通无阻的MNTB，而在AVCN的故障的发生与抑制，并可能是时间处理的计算机制的基础/结果。更一般地说，我们的方法提供了一个正式的工具，用于研究在典型的体内记录条件下具有高统计精度的传输的可靠性。
The giant synapses of Held play an important role in high-fidelity auditory processing and provide a model system for synaptic transmission at central synapses. Whether transmission of action potentials can fail at these synapses has been investigated in recent studies. At the endbulbs of Held in the anteroventral cochlear nucleus (AVCN) a consistent picture emerged, whereas at the calyx of Held in the medial nucleus of the trapezoid body (MNTB) results on the reliability of transmission remain inconsistent. In vivo this discrepancy could be due to the difficulty in identifying failures of transmission. We introduce a novel method for detecting unreliable transmission in vivo. Based on the temporal relationship between a cells' waveform and other potentials in the recordings, a statistical test is developed that provides a balanced decision between the presence and the absence of failures. Its performance is quantified using simulated voltage recordings and found to exhibit a high level of accuracy. The method was applied to extracellular recordings from the synapses of Held in vivo. At the calyces of Held failures of transmission were found only rarely. By contrast, at the endbulbs of Held in the AVCN failures were found under spontaneous, excited, and suppressed conditions. In accordance with previous studies, failures occurred most abundantly in the suppressed condition, suggesting a role for inhibition. Under the investigated activity conditions/anesthesia, transmission seems to remain largely unimpeded in the MNTB, whereas in the AVCN the occurrence of failures is related to inhibition and could be the basis/result of computational mechanisms for temporal processing. More generally, our approach provides a formal tool for studying the reliability of transmission with high statistical accuracy under typical in vivo recording conditions.
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