Expression of estrogen receptor-α and -β mRNAs in human gastric cancer
Expression of estrogen receptor-α and -β mRNAs in human gastric cancer
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DOI：
10.1016/s0304-3835(01)00739-x
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发表时间：
2002-02-25
期刊：
影响因子：
9.7
通讯作者：
Oka, M
中科院分区：
文献类型：
--
作者：
Takano, N;Iizuka, N;Oka, M
为了阐明雌激素受体（ER）在胃癌中的作用，我们采用逆转录-聚合酶链反应（RT-PCR）方法检测了41对胃癌患者的肿瘤组织和非肿瘤组织以及6种胃癌细胞系中ER-α和ER-β mRNA的表达。ER-α和ER-β mRNA分别在41例肿瘤中的21例（51%）和30例（73%）以及41例相应正常组织中的15例（37%）和36例（88%）中检测到。ER-α和/或ER-β mRNA在肿瘤中的表达与临床病理因素之间无统计学显著相关性。在41例肿瘤组织和正常组织中，ER-α和ER-β mRNA的表达在20例（49%）中发生变化，在21例（51%）中无变化。淋巴结转移和肝转移发生率在病变组明显高于无病变组（P = 0.031和P = 0.021）。我们证实ER-α和ER-β mRNA分别在6个胃癌细胞系中的2个和6个中表达。结合这一发现，我们的结果表明ER-β mRNA在胃癌中优先表达。我们的数据还表明，与相应的正常胃组织相比，肿瘤中ER-α和ER-β mRNA表达的改变与胃癌转移潜能的增加有关。ER在胃癌中的作用有待进一步研究。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
To clarify the roles of estrogen receptors (ERs) in gastric cancers, we evaluated expression of ER-alpha and ER-beta mRNAs in 41 pairs of tumorous and non-tumorous tissues of gastric cancer patients and in six gastric cancer cell lines by reverse transcription-polymerase chain reaction method. ER-alpha and ER-beta mRNAs were detected in 21 (51%) and 30 (73%) of 41 tumors and in 15 (37%) and 36 (88%) of 41 corresponding normal tissues, respectively. There were no statistically significant associations between expression of ER-alpha and/or ER-beta mRNAs in tumors and clinicopathologic factors. Between the tumorous and normal tissues, expression of ER-alpha and ER-beta mRNAs were changed in 20 (49%) and unchanged in 21 (51%) of the 41 cases. The incidences of lymph node metastasis and liver metastasis were significantly higher in changed cases than in unchanged cases (P = 0.031 and P = 0.021, respectively). We confirmed that ER-alpha and ER-beta mRNA were expressed in 2 and 6 of the six gastric cancer cell lines, respectively. Together with this finding, our results indicate that ER-beta mRNAs are preferentially expressed in gastric cancers. Our data also suggest that altered expression of ER-alpha and ER-beta mRNAs in tumors compared with corresponding normal gastric tissues is related to increased metastatic potential in gastric cancers. Further studies are needed to clarify the role of ERs in gastric cancers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.