Properties of murine CD8+CD27- T cells
Properties of murine CD8+CD27- T cells
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DOI：
10.1002/eji.200425770
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
van Lier, RAW
中科院分区：
文献类型：
--
作者：
Baars, PA;Sierro, S;van Lier, RAW
在人类中，CD27 表达的丧失与 CD8(+) T 细胞稳定获得效应功能有关。我们发现小鼠CD8(+)CD27(-) T细胞仅限于已引发的CD62L(暗/-)CD44(亮)CCR7(-) T细胞群。淋巴结中不存在CD8(+)CD27(-) T细胞，但在血液、脾脏以及肺和肝等非淋巴器官中可以找到CD8(+)CD27(-) T细胞。原发性流感病毒感染后期，肺和脾中出现低百分比的抗原特异性CD27(-)细胞。从继发性流感病毒感染中恢复后，在肺部发现了高比例的流感特异性CD27（-）T细胞，并且肺部CD8（+）T细胞上CD27的丢失与颗粒酶B的高表达相一致。小鼠巨细胞病毒感染后，病毒特异性CD8+T细胞群上的CD27表达持续丧失，在肝脏和肺中尤其明显。我们认为，在小鼠中，CD27 仅在重复抗原刺激后才从 CD8(+) T 细胞中丢失。此外，CD27(-) T细胞中颗粒酶B和穿孔素的高表达表明，鼠CD8(+) T细胞上CD27的缺乏可用于鉴定具有细胞毒性效应分子表达的记忆T细胞。
In humans, loss of CD27 expression is associated with the stable acquisition of effector functions by CD8(+) T cells. We found that murine CD8(+)CD27(-) T cells were confined to the primed CD62L(dull/-)CD44(bright)CCR7(-) T cell population. CD8(+)CD27(-) T cells were absent from lymph nodes but could be found in blood, spleen and in non-lymphoid organs such as lung and liver. Late after primary influenza virus infection, low percentages of antigen-specific CD27(-) cells emerged in the lung and spleen. After recovery from secondary influenza virus infection, high percentages of influenza-specific CD27(-) T cells were found in the lung and the loss of CD27 on lung CD8(+) T cells coincided with high granzyme B expression. After murine cytomegalovirus infection, loss of CD27 expression on virus-specific CD8(+) T cell populations was sustained and especially marked in liver and lung. We suggest that in mice, CD27 is lost from CD8(+) T cells only after repetitive antigenic stimulation. Moreover, the high expression of both granzyme B and perforin in the CD27(-) T cells suggests that the lack of CD27 on murine CD8(+) T cells can be used to identify memory T cells with expression of cytotoxic effector molecules.