Prognostic impact of Cathepsin B and matrix metalloproteinase-9 in pulmonary adenocarcinomas by immunohistochemical study
Prognostic impact of Cathepsin B and matrix metalloproteinase-9 in pulmonary adenocarcinomas by immunohistochemical study
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DOI：
10.1016/s0169-5002(99)00088-4
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发表时间：
2000-01-01
期刊：
影响因子：
5.3
通讯作者：
Ayabe, H
中科院分区：
文献类型：
--
作者：
Fujise, N;Nanashima, A;Ayabe, H
采用免疫组织学方法检测90例非小细胞肺癌腺癌离体组织中组织蛋白酶B（CB）和基质金属蛋白酶9（MMP-9）的表达情况，探讨其表达程度与临床病理特征的相关性，以探讨肿瘤的发生发展过程。转移。揭示原发病灶基底膜破坏和肿瘤细胞浸润的机制具有重要意义。从 10% 福尔马林固定和石蜡包埋的组织中获得切片。它们与抗人CB多克隆抗体或抗人MMP-9多克隆抗体反应。 90例患者中，分别有58例（64.4%）和39例（48.3）例CB和MMP-9表达阳性。在肿瘤术后复发的患者组织中观察到 CB 表达水平显着升高（P = 0.013）。特别是，在早期 T1N0 病例中也得到了类似的观察结果（P = 0.023）。相反，MMP-9的表达谱中没有表现出这种趋势。此外，比较不同类型转移灶之间的酶表达，CB表达程度较高的患者往往表现出较高的血行转移率和肺内转移率（分别为P = 0.023和P = 0.010）。但MMP-9的表达与患者的预后因素无显着相关性。因此，我们建议评估肿瘤组织中的CB表达可能有助于作为肺腺癌术后预后因素。特别是CB表达较高的T1N0早期癌症病例可能需要术后辅助化疗。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
The expression of Cathepsin B (CB) and matrix metalloproteinase-9 (MMP-9) in extirpated tissues of adenocarcinomas in non-small cell lung cancer from 90 cases was investigated immunohistologically, and the correlations between the extent of the expression and the clinicopathological features were assessed for investigaiting the process of tumor. metastasis. It is important to reveal the mechanisms of destruction of the basal membrane and infiltration of tumor cells at the primary lesion. Sections were obtained from 10%-formalin-fixed and paraffin-embedded tissues. They were reacted with an anti-human CB polyclonal antibody or an anti-human MMP-9 polyclonal antibody. Of 90 patients, 58 (64.4%) and 39 (48.3) cases were found to be positive for CB and MMP-9 expression, respectively. A significantly higher extent of the CB expression was observed in the tissues of patients who showed postoperative recurrence of the tumor (P = 0.013). Especially, a similar observation was obtained among early cases of T1N0 (P = 0.023). In contrast, no such tendency was demonstrated in the expression profile of MMP-9. Furthermore, the enzyme expressions were compared among different types of metastases, Patients with higher extents of CB expression tended to show significantly higher rates of hematogenous and intrapulmonary metastases (P = 0.023 and P = 0.010, respectively). However, there was no significant correlation between MMP-9 expression and the prognostic factor of the patients. Therefore, we suggested that evaluation of CB expression in the tumor tissue might be useful as a postoperative prognostic factor of pulmonary adenocarcinoma. Especially, early cancer of T1N0 cases showing higher expression of CB may need postoperative adjuvant chemotherapy. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.