Biliary excretion of polystyrene microspheres depends on the type of receptor-mediated uptake in rat liver
Biliary excretion of polystyrene microspheres depends on the type of receptor-mediated uptake in rat liver
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DOI：
10.1016/s0304-4165(01)00132-5
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发表时间：
2001-05-03
影响因子：
3
通讯作者：
Kimura, T
中科院分区：
文献类型：
--
作者：
Furumoto, K;Ogawara, K;Kimura, T
本文研究了异硫氰酸荧光素标记的粒径为50 nm的聚苯乙烯微球（MS-50）在大鼠体内的肝摄取和胆汁排泄。肝脏灌注研究表明，不仅apo-E介导的，而且脱唾液酸糖蛋白受体介导的摄取参与MS-50在肝脏中的血清蛋白依赖性摄取的机制。在灌流液中有血清存在的情况下，apo-E介导的MS-50摄取对肝细胞MS-50总摄取的贡献大于通过去唾液酸糖蛋白受体介导的摄取。此外，发现MS-50基本上通过转胞吞作用排泄到胆汁中。MS-50经脱唾液酸糖蛋白受体内吞后被肝细胞胞吐的程度远高于经apo-E介导的摄取。根据这些结果，推测出了可能的调节配体的细胞内分选，这取决于受体介导的摄取机制。(C)2001 Elsevier Science B. V.保留所有权利。
Hepatic uptake and bilialy excretion of fluorescein isothiocyanate-labeled polystyrene microspheres with a particle size of 50 nm (MS-50) were studied in rats. Liver perfusion studies revealed that not only apo-E-mediated but also asialoglycoprotein receptor-mediated uptake is involved in the mechanism of the serum protein-dependent uptake of MS-50 in the liver. The uptake of MS-50 mediated by apo-E contributes more to the total uptake of MS-50 by the hepatocytes than that via asialoglycoprotein receptor in the presence of serum in the perfusate. Furthermore, it was found that MS-50 is substantially excreted into the bile by transcytosis. The extent of exocytosis of MS-50 taken up by the hepatocytes was much higher after MS-50 was endocytosed via asialoglycoprotein receptor than after taken up via the process mediated by apo-E. On the basis of these results, a possible regulation of the intracellular sorting of ligands, depending on the receptor-mediated uptake mechanism, was inferred. (C) 2001 Elsevier Science B.V. All rights reserved.