S1PR1 expression correlates with inflammatory responses to Newcastle disease virus infection
S1PR1 expression correlates with inflammatory responses to Newcastle disease virus infection
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S1PR1 表达与新城疫病毒感染的炎症反应相关。
DOI：
10.1016/j.meegid.2015.10.021
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发表时间：
2016-01-01
影响因子：
3.2
通讯作者：
Ren, Tao
中科院分区：
文献类型：
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作者：
Li, Yaling;Xie, Peng;Ren, Tao
纽卡斯尔病病毒（NDV）是引起鸡纽卡斯尔病的主要病原体。对这种疾病的炎症反应尚未得到很好的表征。以前的研究表明，鞘氨醇-1-磷酸-1受体（S1 PR 1），一种G蛋白偶联受体，在炎症反应的激活中起重要作用。为了更好地了解病毒的发病机制和宿主的炎症反应，我们分析了S1 PR 1在NDV感染过程中的表达。我们观察到鸡胚成纤维细胞（CEF）细胞炎症反应与S1 PR 1表达之间存在直接相关性。强毒NDV感染的CEF细胞也具有升高水平的促炎细胞因子（IL-1 β、IL-6和IL-18）。当使用特异性拮抗剂W146抑制S1 PR 1时，促炎细胞因子的产生下降。S1 PR 1的过表达导致病毒诱导的IL-1 β产生增加。S1 PR 1表达水平对NDV复制无显著影响。这些发现强调了S1 PR 1在NDV感染的炎症反应中的重要作用。(C)2015 Elsevier B. V.版权所有。
Newcastle disease virus (NDV) is the causative agent of Newcastle disease, which is characterized by inflammatory pathological changes in the organs of chickens. The inflammatory response to this disease has not been well characterized. Previous reports showed that the sphingosine-1-phosphate-1 receptor (S1PR1), a G protein-coupled receptor, is important to the activation of inflammatory responses. To understand better the viral pathogenesis and host inflammatory response, we analyzed S1PR1 expression during NDV infection. We observed a direct correlation between chicken embryo fibroblast (CEF) cellular inflammatory responses and S1PR1 expression. Virulent NDV-infected CEF cells also had elevated levels of pro inflammatory cytokines (IL-1 beta, IL-6 and IL-18). When S1PR1 was inhibited by using the specific antagonist W146, pro-inflammatory cytokine production declined. Overexpression of S1PR1 resulted in increased virus-induced IL-1 beta production. S1PR1 expression levels did not impact significantly NDV replication. These findings highlight the important role of S1PR1 in inflammatory responses in NDV infection. (C) 2015 Elsevier B.V. All rights reserved.