Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste
Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste
复制标题
DOI：
10.1016/j.biortech.2010.01.036
复制
发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Yamabhai, Montarop
中科院分区：
文献类型：
--
作者：
Songsiriritthigul, Chomphunuch;Lapboonrueng, Sasithorn;Yamabhai, Montarop
几丁质酶（EC 3.2.1.14）是一种具有多种工业应用的酶。其中包括甲壳素废物的生物转化，甲壳素是甲壳类食品工业中一种高抗性和丰富的生物聚合物，可转化为葡萄糖胺和甲壳低聚糖增值产品。本文报道了地衣芽孢杆菌DSM 8785的几丁质内切酶（ChiA）在大肠杆菌中的表达。大肠杆菌中表达，并对重组酶进行了鉴定。在pH4.0、6.0和9.0、50 ℃条件下，重组ChiA可有效地将胶体几丁质转化为N-乙酰氨基葡萄糖和壳二糖，并在此条件下保持其活性达3天，表明该酶适用于几丁质废物的生物转化。(C)2010爱思唯尔有限公司版权所有。
Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3 days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste. (C) 2010 Elsevier Ltd. All rights reserved.