Cell cycle-related transfer of proteins between the nucleus and cytoplasm of Physarum polycephalum.
Cell cycle-related transfer of proteins between the nucleus and cytoplasm of Physarum polycephalum.
复制标题
多头绒泡菌细胞核和细胞质之间与细胞周期相关的蛋白质转移。
DOI：
10.1016/0014-4827(82)90203-8
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发表时间：
1982
影响因子：
3.7
通讯作者：
Rusch,HP
中科院分区：
文献类型：
--
作者：
JeterJr,JR;Cameron,IL;Hart,NE;Rusch,HP
野生型和多倍体疟原虫的蛋白质。用[~ 3 H]亮氨酸和[~（14）C]亮氨酸预标记多头蚴。然后将两种类型的原质团融合2小时。融合后，分离细胞核，并将较小的野生型细胞核与较大的多倍体细胞核分离。从受体细胞核中分离蛋白质并提取受体核蛋白。不同核蛋白组分中的~ 3 H/~（14）C比值表明，在融合过程中，标记的预制蛋白质从供体细胞差异转移到受体细胞核中。转移的蛋白质的量在不同组分之间以及随着细胞周期的阶段而变化。同位素稀释实验表明，蛋白质转移的这些差异部分是由于核蛋白的高合成率和周转率。
The proteins of wild-type and polyploid plasmodia ofP. polycephalumwere prelabelled with [3H]leucine and [14C]leucine. The two types of plasmodia were then fused for 2 h. Following fusion the nuclei were isolated and the smaller wild-type cell nuclei separated from the larger polyploid cell nuclei. The proteins were isolated from the recipient cell nuclei and the recipient nuclear proteins extracted. Ratios of3H/14C in the various nuclear protein fractions show that during fusion differential transfer of labelled preformed proteins from the donor cell into the recipient cell nucleus occurs. The quantity of proteins transferred varies among the different fractions and with the phase of the cell cycle. Isotopic dilution experiments indicate that these differences in protein transfer are, in part, due to a high rate of synthesis and turnover of the nuclear proteins.