Capping, splicing, and 3′ processing are independently stimulated by RNA polymerase II:: different functions for different segments of the CTD
Capping, splicing, and 3′ processing are independently stimulated by RNA polymerase II:: different functions for different segments of the CTD
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DOI：
10.1101/gad.889101
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发表时间：
2001-07-15
影响因子：
10.5
通讯作者：
Bentley, DL
中科院分区：
文献类型：
--
作者：
Fong, N;Bentley, DL
前体mRNA的加帽、剪接和切割/聚腺苷酸化是相互依赖的事件，它们都在体内由RNA Pol II的羧基末端结构域（CTD）刺激。我们发现CTD独立地增强剪接和3 '加工，并且CTD促进了增强子对剪接的刺激。我们提供的证据表明，CTD对3 '加工的刺激需要与切割刺激因子CstF的50 kD亚基接触。CstF p50的CTD结合结构域的过表达对3 '加工具有显性负效应，而不破坏CstF复合物。CTD包含52个七肽重复序列。CTD羧基末端包括七肽27-52支持加帽、剪接和3 '加工，但氨基末端仅支持加帽。我们得出结论，CTD独立地刺激所有三个主要的前mRNA加工步骤，CTD的不同区域可以在前mRNA加工中发挥不同的功能。
Capping, splicing, and cleavage/polyadenylation of pre-mRNAs are interdependent events that are all stimulated in vivo by the carboxy-terminal domain (CTD) of RNA Pol II. We show that the CTD independently enhances splicing and 3 ' processing and that stimulation of splicing by enhancers is facilitated by the CTD. We provide evidence that stimulation of 3 ' processing by the CTD requires contact with the 50-kD subunit of the cleavage stimulation factor, CstF. Overexpression of the CTD-binding domain of CstF p50 had a dominant-negative effect on 3 ' processing without disrupting the CstF complex. The CTD comprises 52 heptad repeats. The CTD carboxyl terminus including heptads 27-52 supported capping, splicing, and 3 ' processing but the amino terminus supported only capping. We conclude that the CTD independently stimulates ail three major pre-mRNA processing steps and that different regions of the CTD can serve distinct functions in pre-mRNA processing.