The boron requirement and cell wall properties of growing and stationary suspension-cultured Chenopodium album L. cells
The boron requirement and cell wall properties of growing and stationary suspension-cultured Chenopodium album L. cells
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DOI：
10.1104/pp.117.4.1401
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发表时间：
1998-08-01
期刊：
影响因子：
7.4
通讯作者：
Ehwald, R
中科院分区：
文献类型：
--
作者：
Fleischer, A;Titel, C;Ehwald, R
悬浮培养藜细胞能够在缺硼培养基上连续、长期生长。与用硼生长的培养物相比，这些培养物含有更多的扩大和分离的细胞，由于少量细胞的破裂而具有增加的浊度，并且含有具有增加的细胞壁孔径的细胞。这些特性被逆转的硼酸（大于或等于7 μ M）添加到硼缺乏的细胞。C.在100 μ M硼酸存在下生长的白细胞在不进行传代培养时进入稳定期，并保持存活至少3周。从生长相到固定相的过渡伴随着壁孔径的减小。细胞生长无硼酸或与7 μ M硼酸不能减少其壁孔径在过渡到固定相。这些细胞不能在稳定期保持活力，因为它们继续扩张并由于壁破裂而死亡。加入100 μ M硼酸防止壁破裂，壁孔径减小到正常值。我们得出结论，硼是需要保持正常的孔结构的壁矩阵和机械稳定的壁在生长终止。
Suspension-cultured Chenopodiom album L. cells are capable of continuous, long-term growth on a boron-deficient medium. Compared with cultures grown with boron, these cultures contained more enlarged and detached cells, had increased turbidity due to the rupture of a small number of cells, and contained cells with an increased cell wall pore size. These characteristics were reversed by the addition of boric acid (greater than or equal to 7 mu M) to the boron-deficient cells. C. album cells grown in the presence of 100 mu M boric acid entered the stationary phase when they were not subcultured, and remained viable for at least 3 weeks. The transition from the growth phase to the stationary phase was accompanied by a decrease in the wall pore size. Cells grown without boric acid or with 7 mu M boric acid were not able to reduce their wall pore size at the transition to the stationary phase. These cells could not be kept viable in the stationary phase, because they continued to expand and died as a result of wall rupture. The addition of 100 mu M boric acid prevented wall rupture and the wall pore size was reduced to normal values. We conclude that boron is required to maintain the normal pore structure of the wall matrix and to mechanically stabilize the wall at growth termination.