New tropisms of porcine epidemic diarrhoea virus (PEDV) in pigs naturally coinfected by variants bearing large deletions in the spike (S) protein and PEDVs possessing an intact S protein
New tropisms of porcine epidemic diarrhoea virus (PEDV) in pigs naturally coinfected by variants bearing large deletions in the spike (S) protein and PEDVs possessing an intact S protein
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DOI：
10.1111/tbed.13607
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发表时间：
2020-06-03
影响因子：
4.3
通讯作者：
Yamaguchi, Ryoji
中科院分区：
文献类型：
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作者：
Van Diep, Nguyen;Choijookhuu, Narantsog;Yamaguchi, Ryoji
我们先前报道了日本家猪中新型猪流行性腹泻病毒（PEDV）变异体的S蛋白大缺失和具有完整S蛋白的PEDV（S-完整PEDV）的共感染。变异体经常在持续或反复感染的猪场中观察到。为了阐明持续性感染及其向性的变异的作用，我们的遗传特征和免疫化学检测PEDV收集在两个持续性感染的农场的原发性和复发性疫情。我们的结果揭示了在自然感染的猪的肺中，在S蛋白中具有214个氨基酸缺失的PEDV变体和S-完整的PEDV的共感染。鉴定了PEDV的新向性，包括气道的上皮细胞和粘膜下腺体、胆管的上皮细胞和单核细胞/巨噬细胞。这些发现阐明了PEDV感染的机制，流行病学和疾病的模式变化。
We previously reported the coinfection of novel porcine epidemic diarrhoea virus (PEDV) variants bearing large deletions in the S protein and PEDVs possessing an intact S protein (S-intact PEDV) in domestic pigs in Japan. The variants were frequently observed in pig farms with persistent or recurrent infection. To elucidate the role of the variants in persistent infections and their tropism properties, we genetically characterized and immunohistochemically detected PEDVs collected in primary and recurrent outbreaks in two persistently infected farms. Our results revealed coinfection of the PEDV variants bearing a 214-amino acid deletion in the S protein and S-intact PEDVs in the lungs of the naturally infected pigs. New tropisms of PEDV, including epithelial cells and submucosal glands of the airway tract, epithelial cells of the bile duct, and monocytes/macrophages were identified. The findings elucidate the mechanism of PEDV infection, epidemiology and pattern changes in the disease.