A new step toward the artificial ovary: survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold
A new step toward the artificial ovary: survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold
复制标题
DOI：
10.1016/j.fertnstert.2013.12.025
复制
发表时间：
2014-04-01
影响因子：
6.7
通讯作者：
Amorim, Christiani Andrade
中科院分区：
文献类型：
--
作者：
Luyckx, Valerie;Dolmans, Marie-Madeleine;Amorim, Christiani Andrade
目的：制备人工卵巢，为因恶性肿瘤复发而无法从卵巢冷冻组织移植中获益的患者提供另一种恢复生育能力的方法。设计：体内实验研究。单位：大学附属医院妇科研究单位。动物：6周龄雌性NMRI小鼠。干预措施：自体移植分离的腔前卵泡和卵巢细胞（OCs），包裹在两种纤维蛋白基质中，其中含有低浓度的纤维蛋白原（F; mg/mL）和凝血酶（T;IU/mL）： F12.5/T1和F25/T4。主要观察指标：卵泡密度和发育、OC存活和增殖、炎症反应和血管化。结果：1周后，卵泡恢复率为30.8% (F25/T4) ~ 31.8% （F12.5/T1）。通过末端脱氧核苷酸转移酶介导的dUTP nickend标记试验证明，这两种纤维蛋白制剂的所有卵泡都是活的或损伤最小，并且在生长阶段（原发性、继发性和窦性卵泡），Ki67免疫染色证实了这一点。离体oc在移植后也能存活和增殖，如
Objective: To create an artificial ovary to provide an alternative way of restoring fertility in patients who cannot benefit from transplantation of cryopreserved ovarian tissue due to the threat of reintroducing malignant cells.Design: In vivo experimental study.Setting: Gynecology research unit in a university hospital.Animal(s): Six-week-old female NMRI mice.Intervention(s): Autografting of isolated preantral follicles and ovarian cells (OCs) encapsulated in two fibrin matrices containing low concentrations of fibrinogen (F; mg/mL) and thrombin (T;IU/mL): F12.5/T1 and F25/T4.Main Outcome Measure(s): Follicular density and development, OC survival and proliferation, inflammatory response, and vascularization.Result(s): After 1 week, the follicle recovery rate ranged from 30.8% (F25/T4) to 31.8% (F12.5/T1). With both fibrin formulations, all follicles were found to be alive or minimally damaged, as demonstrated by terminal deoxynucleotide transferase- mediated dUTP nickend labeling assay, and at the growing stage (primary, secondary, and antral follicles), confirmed by Ki67 immunostaining. Isolated OCs also survived and proliferated after grafting, as evidenced by