Development of a micro-planar Ag/AgCl quasi-reference electrode with long-term stability for an amperometric glucose sensor
Development of a micro-planar Ag/AgCl quasi-reference electrode with long-term stability for an amperometric glucose sensor
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DOI：
10.1016/s0003-2670(02)00334-3
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发表时间：
2002-07-01
影响因子：
6.2
通讯作者：
Ito, N
中科院分区：
文献类型：
--
作者：
Matsumoto, T;Ohashi, A;Ito, N
本文报道了一种具有长期稳定性的微平面Ag/AgCl准参比电极（QRE），它具有长期电位稳定性和对干扰物质的实际免疫性，并已应用于电流型葡萄糖传感器的血糖测定。为了制造，我们首先用γ-氨丙基三乙氧基硅烷（γ-APTES）然后用全氟化碳聚合物（PFCP）涂覆银/氯化银（Ag/AgCl）电极。测试证明了新电极在150 mM KCl中保持82天稳定的能力，并且还显示了其对干扰物质（1 mM KI和1 mM KBr）、pH和血清的影响的不渗透性。此外，在血浆样品中葡萄糖浓度的测试中，临床分析仪获得的结果与使用所开发的Ag/AgCl QRE的电流型葡萄糖传感器获得的结果之间的良好相关系数为0.954（n = 30，Y = 1.02X + 0.20），表明Ag/AgCl QRE作为血浆样品的参比电极功能良好。(C)2002 Elsevier Science B. V.保留所有权利。
This paper reports a micro-planar Ag/AgCl quasi-reference electrode (QRE) with long-term stability which is characterized by both long-term potential stability and practical immunity to interference species, and which has been applied for use with an amperometric glucose sensor for plasma glucose. For fabrication, we coated a silver/silver chloride (Ag/AgCl) electrode first with gamma-aminopropyltriethoxysilane (gamma-APTES) and then with perfluorocarbon polymer (PFCP). Tests demonstrate the new electrode's ability to remain stable over an 82-day period in 150 mM KCl, and also show its imperviousness to the effects of interference species (1 mM KI and 1 mM KBr), pH, and serum. Furthermore, in tests for glucose concentrations in plasma samples, a good correlation coefficient, 0.954 (n = 30, Y = 1.02X + 0.20), was demonstrated between results obtained with a clinical analyzer and those obtained with an amperometric glucose sensor that used the developed Ag/AgCl QRE, showing that the Ag/AgCl QRE functions well as a reference electrode for plasma samples. (C) 2002 Elsevier Science B.V. All rights reserved.