Adenosine detection by using gold nanoparticles and designed aptamer sequences
Adenosine detection by using gold nanoparticles and designed aptamer sequences
复制标题
使用金纳米颗粒和设计的适体序列检测腺苷。
DOI：
10.1039/b900900k
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Li, Fan;Zhang, Juan;Fan, Chunhai
基于金纳米粒子（AuNPs）和基因工程DNA适配体，我们设计了一种新的生物检测策略，用于检测腺苷这一小分子靶物质。在该设计中，适体被工程化为由两段随机卷曲样ssDNA组成，这两段ssDNA分别通过它们的5 '-巯基修饰的末端连接到AuNP。它们可以重新组装成完整的适体三级结构，并在特异性靶标存在下诱导纳米颗粒聚集。结果表明，金纳米粒子可以有效地区分这两种不同的DNA结构，通过其特征的表面等离子体共振为基础的颜色变化。用这种方法，腺苷可以选择性地检测在低微摩尔范围内，这意味着这里报告的策略可以适用于检测其他几个小的目标分子。
Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 5'-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules.