Taste cell formation does not require gustatory and somatosensory innervation
Taste cell formation does not require gustatory and somatosensory innervation
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DOI：
10.1016/j.neulet.2010.01.039
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发表时间：
2010-03-08
影响因子：
2.5
通讯作者：
Nosrat, Christopher A.
中科院分区：
文献类型：
--
作者：
Ito, Akira;Nosrat, Irina V.;Nosrat, Christopher A.
味蕾和味乳头对神经支配的依赖性一直是争论很久的问题。以往的研究表明，神经营养因子脑源性神经营养因子(BDNF)和神经营养因子-3(NT-3)在舌味觉和体感神经支配的建立中起着重要作用。BDNF缺失突变小鼠在味觉神经支配和味蕾缺失方面表现出严重的缺陷，而NT-3缺失突变则减少了舌乳头的体感神经支配。这些结果证明BDNF或NT-3零突变分别影响不同的感觉方式(即味觉和躯体感觉)。在这项研究中，我们分析了BDNF x NT-3双基因敲除小鼠的味蕾发育，以探讨味蕾发育与味觉/体感神经支配的关系。我们的结果表明，在最初阶段，在神经纤维到达乳头中的适当区域之前，味蕾的形成不需要神经支配。然而，在突触形成阶段，神经纤维分支进入顶端上皮后，需要神经支配，并在味蕾/乳头的发育中发挥重要作用。(C)2010爱思唯尔爱尔兰有限公司。保留所有权利。
Dependency of taste buds and taste papillae on innervation has been debated for a long time. Previous research showed neurotrophins, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), play an important role for the establishment of the lingual gustatory and somatosensory innervation. BDNF null mutant mice showed severe deficits in gustatory innervation and loss of taste buds while NT-3 null mutation reduced lingual somatosensory innervation to tongue papillae. These results proved BDNF or NT-3 null mutations affected different sensory modalities (i.e. gustatory and somatosensory, respectively). In this Study, we analyzed taste bud development in BDNF x NT-3 double knockout mice to examine the relationship between taste bud development and gustatory/somatosensory innervation. Our results demonstrate that, at the initial stage, before nerve fibers reached the appropriate areas in the papilla, taste bud formation did not require innervation. However, at the synaptogenic stage, after nerve fibers ramified into the apical epithelium, innervation was required and played an essential role in the development of taste buds/papillae. (C) 2010 Elsevier Ireland Ltd. All rights reserved.