UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions.
UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions.
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DOI：
10.1093/nar/28.2.e3
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发表时间：
2000-01-15
影响因子：
14.9
通讯作者：
Ge, H
中科院分区：
文献类型：
--
作者：
Ge, H
蛋白质-蛋白质相互作用已被广泛应用于基因表达途径的研究，有望成为药物开发的新途径。在这里，我报告了通用蛋白质阵列(UPA)系统的发展，该系统提供了一种灵敏、定量、多用途、有效和简单的技术，不仅可以确定特定的蛋白质-蛋白质相互作用，而且还可以确定蛋白质与DNA、RNA、配体和其他小化学物质的特定相互作用。(I)由于使用了纯化的蛋白质，因此结果很容易解释。(Ii)uPA可多次用于不同的目标，使大多数实验室、医院和生物技术公司在经济上负担得起。(Iii)与DNA芯片或DNA微阵列不同，不需要额外的仪器。(4)由于uPA使用活性蛋白质(没有变性和复性)，与大多数现有方法相比，它更敏感。(V)由于uPA可以在一次实验中分析数百种(甚至在一个蛋白质微阵列上分析数千种)蛋白质，因此它是一种非常有效的方法来筛选蛋白质作为癌症和其他人类疾病的药物靶点。
Protein-protein interactions have been widely used to study gene expression pathways and may be considered as a new approach to drug discovery. Here I report the development of a universal protein array (UPA) system that provides a sensitive, quantitative, multi-purpose, effective and easy technology to determine not only specific protein-protein interactions, but also specific interactions of proteins with DNA, RNA, ligands and other small chemicals. (i) Since purified proteins are used, the results can be easily interpreted. (ii) UPA can be used multiple times for different targets, making it economically affordable for most laboratories, hospitals and biotechnology companies. (iii) Unlike DNA chips or DNA microarrays, no additional instrumentation is required. (iv) Since the UPA uses active proteins (without denaturation and renaturation), it is more sensitive compared with most existing methods. (v) Because the UPA can analyze hundreds (even thousands on a protein microarray) of proteins in a single experiment, it is a very effective method to screen proteins as drug targets in cancer and other human diseases.