Isolation and characterization of Pseudoalteromonas strains with divergent polycyclic aromatic hydrocarbon catabolic properties
Isolation and characterization of Pseudoalteromonas strains with divergent polycyclic aromatic hydrocarbon catabolic properties
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DOI：
10.1111/j.1462-2920.2005.00871.x
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Staley, JT
中科院分区：
文献类型：
--
作者：
Hedlund, BP;Staley, JT
15株多环芳烃（PAH）降解菌，经鉴定为交替假单胞菌属（Pseudoalteromonasspp.）从Eagle Harbor，Puget Sound，USA分离，使用最可能数程序，其中萘或菲是唯一的碳源和能源。尽管有相同的16 S rDNA序列，一些分解代谢许多多环芳烃，而其他人只氧化萘。一个假定的萘1，2-双加氧酶基因片段的聚合酶链反应扩增的萘降解菌株，并显示几乎相同的基因存在于Neptunomonasnaphthovorans，表明水平转移。
Fifteen strains of polycyclic aromatic hydrocarbon (PAH)-catabolizing bacteria, identified as Pseudoalteromonas spp. were isolated from Eagle Harbor, Puget Sound, USA, using a most probable number procedure in which naphthalene or phenanthrene was the sole carbon and energy source. Despite having identical 16S rDNA sequences, some catabolized many PAHs, whereas others oxidized only naphthalenes. A putative naphthalene 1,2-dioxygenase gene fragment was polymerase chain reaction-amplified from the naphthalene-degrading strains and shown to be almost identical to a gene present in Neptunomonas naphthovorans, suggesting horizontal transfer.