Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells.
Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells.
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DOI：
10.3791/54785
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发表时间：
2016-12-13
期刊：
影响因子：
--
通讯作者：
Schwarzacher W
中科院分区：
文献类型：
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作者：
Correia Carreira S;Armstrong JP;Okuda M;Seddon AM;Perriman AW;Schwarzacher W
许多重要的生物医学应用，如细胞成像和远程操作，可以通过超顺磁性氧化铁纳米颗粒(SPION)标记细胞来实现。实现细胞对SPIONS的充分摄取是一项挑战，传统上通过将细胞暴露在高浓度的SPIONS中或延长暴露时间(长达72小时)来应对。然而，这些策略可能会调解毒性。在这里，我们介绍了基于蛋白质的Spion磁铁蛋白的合成以及一种简单的表面功能化方案，该方案能够在低暴露浓度下实现快速细胞磁化。磁性铁蛋白的Spion核由钴掺杂的氧化铁组成，其平均粒径为8.2 nm，矿化在马脾脱铁蛋白的腔内。磁铁蛋白的化学阳离子作用产生了一种新型的、膜活性很高的SPION，它可以磁化人骨髓间充质干细胞(HMSCs)，孵育时间短至一分钟，铁浓度低至0.2 mM。
Many important biomedical applications, such as cell imaging and remote manipulation, can be achieved by labeling cells with superparamagnetic iron oxide nanoparticles (SPIONs). Achieving sufficient cellular uptake of SPIONs is a challenge that has traditionally been met by exposing cells to elevated concentrations of SPIONs or by prolonging exposure times (up to 72 hr). However, these strategies are likely to mediate toxicity. Here, we present the synthesis of the protein-based SPION magnetoferritin as well as a facile surface functionalization protocol that enables rapid cell magnetization using low exposure concentrations. The SPION core of magnetoferritin consists of cobalt-doped iron oxide with an average particle diameter of 8.2 nm mineralized inside the cavity of horse spleen apo-ferritin. Chemical cationization of magnetoferritin produced a novel, highly membrane-active SPION that magnetized human mesenchymal stem cells (hMSCs) using incubation times as short as one minute and iron concentrations as lows as 0.2 mM.