Imaging Structural and Electrical Changes of Aging Cells Using Scanning Ion Conductance Microscopy.
Imaging Structural and Electrical Changes of Aging Cells Using Scanning Ion Conductance Microscopy.
复制标题
使用扫描离子电导显微镜对老化细胞的结构和电变化进行成像。
DOI：
10.1002/smtd.202301315
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发表时间：
2023
期刊：
影响因子：
12.4
通讯作者：
Ning Gu
中科院分区：
文献类型：
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作者：
Yao Song;Shuting Zhang;Chen Cao;Jia Yan;Mei Li;Xinyu Li;Feng Chen;Ning Gu
细胞外膜的局部电荷密度和分布在各种细胞过程中起着至关重要的作用，例如膜蛋白的调节和定位、电生理信号转导、转录控制、细胞生长和细胞死亡。在这项研究中，采用一种基于扫描离子电导显微镜的新型方法来进行细胞外膜绘图。该方法不仅可以可视化单个电池周围的动态形貌和表面电荷分布，还可以区分电荷差异。为了验证该方法的准确性和有效性，首先操纵模型样品表面的电荷密度，随后探索使用有限元建模（FEM）的电荷传感机制。通过应用这种方法，可以监测正常和衰老人牙髓干细胞（hDPSC）的细胞外电荷分布和形貌结构。有趣的是，观察到细胞衰老后表面电荷明显变得更负。这种创新方法使我们能够获得对细胞衰老过程中表面电荷变化的宝贵见解，这有助于更好地了解与年龄相关的疾病的潜在机制和潜在治疗策略。
The local charge density and distribution of extracellular membranes play a crucial role in the various cellular processes, such as regulation and localization of membrane proteins, electrophysiological signal transduction, transcriptional control, cell growth, and cell death. In this study, a novel scanning ion conductance microscopy-based method is employed to extracellular membrane mapping. This method allows to not only visualize the dynamic topography and surface charge distribution around individual cells, but also distinguish the charge difference. To validate the accuracy and effectiveness of this method, the charge density on model sample surfaces are initially manipulated and the charge sensing mechanism using finite element modeling (FEM) is explored subsequently. By applying this method, both the extracellular charge distributions and topography structures of normal and senescent human dental pulp stem cells (hDPSCs) are able to monitor. Interestingly, it is observed that the surface charge became significantly more negative after cellular senescence. This innovative approach enables us to gain valuable insights into surface charge changes during cellular senescence, which can contribute to a better understanding of the underlying mechanisms and potential therapeutic strategies for age-related diseases.
DOI：
10.1101/2020.08.26.267849
发表时间：
2020-08
期刊：
bioRxiv
影响因子：
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作者：
Kelsey Cremin;Bryn A. Jones;J. Teahan;Gabriele Meloni;David Perry;Christian Zerfaß;Munehiro Asally-Munehiro-Asall
通讯作者：
Kelsey Cremin;Bryn A. Jones;J. Teahan;Gabriele Meloni;David Perry;Christian Zerfaß;Munehiro Asally-Munehiro-Asall