Molecular Dissection of Phagocytosis by Proteomic Analysis in Entamoeba histolytica.
Molecular Dissection of Phagocytosis by Proteomic Analysis in Entamoeba histolytica.
复制标题
作者：
溶组织内阿米巴是引起阿米巴病的肠道原虫。溶组织埃希氏菌的滋养体吞噬肠道和其他器官中的人体细胞，这是其发病机制的标志。吞噬和巨噬细胞增多是其毒力的关键生物学功能，也有助于从环境中吸收营养物质的增殖。我们以前阐明了与吞噬和巨噬细胞增多症相关的各种蛋白质的作用，包括Rab小GTP酶、Rab效应物(包括逆转聚体)、肌醇磷脂结合蛋白、溶酶体水解酶受体、蛋白激酶和细胞骨架蛋白。然而，一些参与吞噬和巨噬细胞增多症的蛋白质仍有待鉴定，它们参与的机制细节必须在分子水平上阐明。到目前为止，已经进行了一系列与吞噬小体相关并可能参与吞噬作用的蛋白质的研究。在这篇综述中，我们回顾了我们以前进行的所有吞噬体蛋白质组研究，以重申关于吞噬体蛋白质组的信息。我们展示了构成吞噬体蛋白的核心集合，以及仅以瞬时或条件依赖的方式招募的吞噬体蛋白质集合。这些分析得到的吞噬小体蛋白质组目录可作为未来机制研究的有用信息来源，也可用于确认或排除各种研究中感兴趣的蛋白质是否可能或可能参与吞噬细胞和吞噬小体生物发生的可能性。
Entamoeba histolytica is the enteric protozoan parasite responsible for amebiasis. Trophozoites of E. histolytica ingest human cells in the intestine and other organs, which is the hallmark of its pathogenesis. Phagocytosis and trogocytosis are pivotal biological functions for its virulence and also contribute to the proliferation of nutrient uptake from the environment. We previously elucidated the role of a variety of proteins associated with phagocytosis and trogocytosis, including Rab small GTPases, Rab effectors, including retromer, phosphoinositide-binding proteins, lysosomal hydrolase receptors, protein kinases, and cytoskeletal proteins. However, a number of proteins involved in phagocytosis and trogocytosis remain to be identified, and mechanistic details of their involvement must be elucidated at the molecular level. To date, a number of studies in which a repertoire of proteins associated with phagosomes and potentially involved in phagocytosis have been conducted. In this review, we revisited all phagosome proteome studies we previously conducted in order to reiterate information on the proteome of phagosomes. We demonstrated the core set of constitutive phagosomal proteins and also the set of phagosomal proteins recruited only transiently or in condition-dependent fashions. The catalogs of phagosome proteomes resulting from such analyses can be a useful source of information for future mechanistic studies as well as for confirming or excluding a possibility of whether a protein of interest in various investigations is likely or is potentially involved in phagocytosis and phagosome biogenesis.
登录
查看更多内容
影响因子：
5.7
作者：
通讯作者：
--
影响因子：
3.4
作者：
Bharadwaj, Ravi;Sharma, Shalini;Bhattacharya, Alok
通讯作者：
Bhattacharya, Alok
影响因子：
3.3
作者：
Nakada-Tsukui, K;Saito-Nakano, Y;Nozaki, T
通讯作者：
Nozaki, T
影响因子：
3.4
作者：
Mitra, Biswa Nath;Saito-Nakano, Yumiko;Nozaki, Tomoyoshi
通讯作者：
Nozaki, Tomoyoshi
影响因子：
6.4
作者：
Matthiesen J;Bär AK;Bartels AK;Marien D;Ofori S;Biller L;Tannich E;Lotter H;Bruchhaus I
通讯作者：
Bruchhaus I