Phylogenetic analysis of developmental and postnatal mouse cell lineages.
Phylogenetic analysis of developmental and postnatal mouse cell lineages.
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DOI：
10.1111/j.1525-142x.2009.00393.x
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发表时间：
2010-01
影响因子：
2.9
通讯作者：
Horwitz MS
中科院分区：
文献类型：
--
作者：
Salipante SJ;Kas A;McMonagle E;Horwitz MS
命运图描绘了细胞如何通过过去的谱系关系联系在一起，是研究发育和体细胞过程的有用工具。然而，利用现有的技术，还不可能生成复杂生物体（如小鼠）的详细命运图。因此，我们和其他人提出了一种新的方法，“系统发育命运映射”，其中动物个体细胞携带的体细胞突变模式用于通过系统发育推断来追溯性地推断谱系关系。在这里，我们对从一只小鼠中分离的近300个细胞的324个易突变的聚鸟嘌呤束的基因组多态性进行了编目，并通过遗传学和基于网络的方法探索了细胞的谱系关系。我们提出了一个小鼠胚胎发生模型，其中大量的细胞混合的早期阶段，随后更连贯的克隆生长。我们发现，来自某些组织的细胞在其他特定组织中的近亲数量比预期的要多，这表明这些细胞群来自一个类似的祖先谱系库。最后，我们研究了出生后组织中细胞更新的动态（细胞丢失和替换的频率）。这项工作提供了一个纵向研究的发展谱系，从概念到成年，并提供洞察小鼠胚胎学的基本问题，以及出生后发生的体细胞过程。
Fate maps depict how cells relate together through past lineage relationships, and are useful tools for studying developmental and somatic processes. However, with existing technologies, it has not been possible to generate detailed fate maps of complex organisms such as the mouse. We and others have therefore proposed a novel approach, “phylogenetic fate mapping,” where patterns of somatic mutation carried by the individual cells of an animal are used to retrospectively deduce lineage relationships through phylogenetic inference. Here, we have cataloged genomic polymorphisms at 324 mutation-prone polyguanine tracts for nearly 300 cells isolated from a single mouse, and have explored the cells’ lineage relationships both phylogenetically and through a network-based approach. We present a model of mouse embryogenesis, where an early period of substantial cell mixing is followed by more coherent growth of clones later. We find that cells from certain tissues have greater numbers of close relatives in other specific tissues than expected from chance, suggesting that those populations arise from a similar pool of ancestral lineages. Finally, we have investigated the dynamics of cell turnover (the frequency of cell loss and replacement) in postnatal tissues. This work offers a longitudinal study of developmental lineages, from conception to adulthood, and provides insight into basic questions of mouse embryology as well as the somatic processes that occur after birth.
影响因子：
1.8
作者：
Thompson, James M;Salipante, Stephen J
通讯作者：
Salipante, Stephen J