Identifying chemical changes in subchondral bone taken from murine knee joints using Raman spectroscopy
Identifying chemical changes in subchondral bone taken from murine knee joints using Raman spectroscopy
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DOI：
10.1366/000370206778664743
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发表时间：
2006-10-01
影响因子：
3.5
通讯作者：
Morris, Michael D.
中科院分区：
文献类型：
--
作者：
Dehring, Karen A.;Crane, Nicole J.;Morris, Michael D.
介绍了拉曼光谱在软骨下骨分析中的应用。软骨健康对软骨下骨的影响已广泛应用放射学和组织学方法进行研究；然而，没有直接测定矿物成分的方法。我们展示了股骨髁的拉曼光谱，并观察了来自软骨下骨的矿物带。在两个单独的实验中，将转基因小鼠模型的早发性骨关节炎（OA）和脂肪萎缩与野生型小鼠组织进行比较。拉曼光谱用于鉴定软骨下骨矿物质的化学变化，这些变化可能伴随或先于组织学观察到的形态变化。将转基因小鼠与年龄匹配的野生型小鼠进行比较。利用已建立的拉曼光谱标记，如碳酸盐-磷酸盐比、矿物-基质比（MTMR）和酰胺I比，观察矿物或胶原基质的细微变化。拉曼显微镜配置可以快速收集位于完整的非矿化关节软骨层下的矿化层的拉曼光谱。讨论了软骨层对光谱采集的影响。提出的技术能够在分子水平上深入了解发生在软骨下骨中的化学变化。
Application of Raman spectroscopy to analysis of subchondral bone is described. The effect of cartilage health on subchondral bone has been widely studied using radiological and histological methods; however, there is no method to directly assay mineral components. We present Raman spectra of femur condyles and observe mineral bands that arise from the subchondral bone. In two separate experiments, transgenic mouse models of early-onset osteoarthritis (OA) and lipoatrophy were compared to tissue from wild-type mice. Raman spectroscopy was used to identify chemical changes in the mineral of subchondral bone that may accompany or precede morphological changes that can be observed by histology. The transgenic mice were compared to age-matched wild-type mice. Subtle alterations in the mineral or collagen matrix were observed by Raman spectroscopy using established Raman markers such as the carbonate-to-phosphate ratio, mineral-to-matrix ratio (MTMR), and amide I ratio. The Raman microscope configuration enabled rapid collection of Raman spectra from the mineralized layer that lies under an intact layer of non-mineralized articular cartilage. The effect of the cartilage layer on collection of spectra is discussed. The technique proposed is capable of providing insight into the chemical changes that occur in subchondral bone on a molecular level.