Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules
Rhizobium etli is able to emit nitrous oxide by connecting assimilatory nitrate reduction with nitrite respiration in the bacteroids of common bean nodules
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DOI：
10.1080/17429145.2023.2251511
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发表时间：
2023-12-31
影响因子：
3.2
通讯作者：
Delgado，Maria J.
中科院分区：
文献类型：
--
作者：
Hidalgo-Garcia，Alba;Tortosa，German;Delgado，Maria J.
豆科植物可以直接通过一些根瘤菌物种排放有效的温室气体一氧化二氮（N2 O），这些根瘤菌物种能够在自由生活条件下与植物共生。本研究首次证实了菜豆-根瘤菌共生体对NO3-的响应释放N2 O的能力。我们发现，细菌同化硝酸还原酶（NarB）以及亚硝酸还原酶（NirK）和一氧化氮还原酶（cNor）还原酶有助于一氧化氮（NO）和N2 O的形成在根瘤。我们还证明了R. etliNarK参与NO2-排出，并将细胞质中NarB的NO3-还原与周质中的NirK和cNor反硝化活性联系起来。这项工作产生的知识将有助于探索农业土壤管理的战略和可持续做法，以增加豆类作物产量和减少温室气体排放。
Legumes can contribute to emissions of the potent greenhouse gas nitrous oxide (N2O) directly by some rhizobia species that are able to denitrify under free-living conditions and in symbiotic association with the plant. In this study, the capacity ofPhaseolus vulgaris-Rhizobium etlisymbiosis to emit N2O in response to nitrate (NO3-) has been demonstrated for the first time. We found that bacteroidal assimilatory nitrate reductase (NarB) as well as nitrite reductase (NirK) and nitric oxide reductase (cNor) denitrifying enzymes contribute to nitric oxide (NO) and N2O formation in nodules. We also show thatR. etliNarK is involved in NO2-extrusion and links NO3-reduction by NarB in the cytoplasm with NirK and cNor denitrification activities in the periplasm. The knowledge generated in this work will be instrumental for exploring strategies and sustainable practices in agricultural soil management to increase legume crop yield and mitigate greenhouse gas emissions.