Production of manganese peroxidase by pellet culture of the lignin-degrading basidiomycete, Pleurotus ostreatus
Production of manganese peroxidase by pellet culture of the lignin-degrading basidiomycete, Pleurotus ostreatus
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DOI：
10.1007/s002530100653
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发表时间：
2001-06-01
影响因子：
5
通讯作者：
Kuwahara, M
中科院分区：
文献类型：
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作者：
Ha, HC;Honda, Y;Kuwahara, M
在葡萄糖/蛋白胨/麦麸培养基中搅拌培养，平菇42号产生木质素分解酶、锰过氧化物酶（MnP）和漆酶。形成直径1-2毫米的菌丝球对MnP的生产至关重要；培养基中溶解氧的浓度对MnP的生产有很大影响，浓度超过5 ppm是MnP生产所必需的。MnP活性在培养第7-9天，即消耗营养葡萄糖后达到最大值。从培养开始就引入氧气，导致形成大颗粒，导致MnP活性水平低。在搅拌培养中，P. ostreatus No. 42产生了两种MnP同工酶。主要同工酶F-2为36.4 kDa， pi为3.95。该同工酶的MnP特性、K值、对Mn2+的依赖性和最适pH值与不同培养条件下产生的mnp3具有相似性。n端氨基酸序列分析表明F-2与mnp3非常相似。
Pleurotus ostreatus No. 42 produced the ligninolytic enzymes, manganese peroxidase (MnP) and laccase, in agitation culture in glucose/peptone/wheatbran medium. Formation of mycelial pellets 1-2 mm in diameter was essential for the production of MnP: and the concentration of dissolved oxygen in the culture medium greatly influenced the production of MnP, a concentration over 5 ppm being necessary for MnP production. The maximal activity of MnP was obtained on days 7-9 of culture, after the consumption of nutrient glucose. Introduction of oxygen from the start of the cultivation caused large pellet formation, which resulted in a low MnP activity level. P. ostreatus No. 42 produced two MnP isozymes in agitation culture. The major isozyme, F-2, was 36.4 kDa and had a pi of 3.95. The MnP characteristics, K, values, dependence on Mn2+ and optimum pH showed the similarity between this isozyme and MnP 3, which was produced under different culture conditions. Analysis of the N-terminal amino acid sequence indicated the close similarity of F-2 to MnP 3.