Endogenous gpdh-1 transcriptional reporters as new tools for the study of the osmotic stress response.
Endogenous gpdh-1 transcriptional reporters as new tools for the study of the osmotic stress response.
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DOI：
10.17912/micropub.biology.000818
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发表时间：
2023
影响因子：
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通讯作者：
Lamitina, Todd
中科院分区：
文献类型：
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作者：
Veroli, Maria Victoria;Lamitina, Todd
使用标准的转录报告基因在体内监测gpdh-1基因的表达是一个强有力的和常用的工具，在C.优雅像所有的转基因报道基因一样，这些gpdh-1报道基因也有重要的局限性，限制了它们的应用。为了克服这些限制，我们使用CRISPR/Cas9方法创建了三种不同的gpdh-1报告基因，以将GFP的几种变体插入内源性gpdh-1基因座中。这些新菌株为分析gpdh-1的调控通路提供了一个更强大和准确的工具。
In vivo monitoring of gpdh-1 gene expression using standard transcriptional reporters is a powerful and commonly used tool for genetic dissection of the osmotic stress response in C. elegans . Like all transgene reporters, these gpdh-1 reporters have important limitations that restrict their utility. To overcome these limitations, we created three different gpdh-1 reporters using CRISPR/Cas9 methods to insert several variants of GFP into the endogenous gpdh-1 locus. These new strains provide a more powerful and accurate tool for the analysis of gpdh-1 regulatory pathways.