The association between gut microbiome and growth in infants with cystic fibrosis.
The association between gut microbiome and growth in infants with cystic fibrosis.
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肠道微生物组与囊性纤维化婴儿生长之间的关联。
DOI：
10.1016/j.jcf.2023.08.001
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Davis,SD
中科院分区：
文献类型：
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作者：
Deschamp,AR;Chen,Y;Wang,WF;Rasic,M;Hatch,J;Sanders,DB;Ranganathan,SC;Ferkol,T;Perkins,D;Finn,P;Davis,SD
背景在囊性纤维化（CF）中，胃肠道的病理生理变化导致营养不良和肠道微生物组改变。微生物组的改变与线性生长、肠道炎症和呼吸道症状有关。阐明这些肠道微生物组的变化可能有助于了解 CF 未来的营养管理。方法在美国 (US-CF) 和澳大利亚 (AUS-CF) 的四个地点对婴儿进行了 12 个月的随访。对纵向粪便样本进行 16S rRNA 基因测序。使用广义线性混合模型评估微生物丰度与年龄、抗生素预防、营养不良和母乳喂养之间的关联。对各组之间的分类学和预测功能特征进行比较。结果患有 CF 的婴儿 (N = 78) 被纳入一项更大规模研究的一部分。 AUS-CF 婴儿的平均年龄别体重 z 得分高于 US-CF 婴儿 (p= 0.02)。一部分参与者（CFN = 40，非 CF 疾病对照 N = 10）提供了粪便样本用于微生物组分析。与 US-CF 婴儿相比，AUS-CF 婴儿的粪便 α 多样性较低 (p< 0.001)。与 US-CF 婴儿相比，AUS-CF 婴儿的粪便变形菌相对丰度更高，这与抗生素预防相关（p < 0.001）。营养不良（年龄别体重<10%）与乳球菌耗尽相关（p<0.001）。抗生素预防 (p= 0.002) 和营养不良 (p= 0.012) 与负责短链脂肪酸加工的代谢途径的预计活性降低有关。 结论 在患有 CF 的婴儿中，两大洲的肠道微生物组组成和多样性存在差异。肠道微生物多样性与生长无关。营养不良和抗生素预防与 SCFA 发酵减少之间的关系可能会对肠道健康和功能产生影响，值得进一步研究。
BackgroundIn cystic fibrosis (CF), pathophysiologic changes in the gastrointestinal tract lead to malnutrition and altered gut microbiome. Microbiome alterations have been linked to linear growth, gut inflammation and respiratory manifestations. Elucidating these gut microbiome alterations may provide insight into future nutritional management in CF.MethodsInfants were followed for 12-months at four sites in the United States (US-CF) and Australia (AUS-CF). 16S rRNA gene sequencing was performed on longitudinal stool samples. Associations between microbial abundance and age, antibiotic prophylaxis, malnutrition, and breast feeding were evaluated using generalized linear mixed models. Taxonomic and predictive functional features were compared between groups.ResultsInfants with CF (N= 78) were enrolled as part of a larger study. AUS-CF infants had higher mean weight-for-age z-scores than US-CF infants (p= 0.02). A subset of participants (CFN= 40, non-CF disease controlsN= 10) provided stool samples for microbiome analysis. AUS-CF infants had lower stool alpha diversity compared to US-CF infants (p< 0.001). AUS-CF infants had higher relative abundance of stool Proteobacteria compared to US-CF infants which was associated with antibiotic prophylaxis (p< 0.001). Malnutrition (weight-for-age <10th percentile) was associated with depletedLactococcus(p< 0.001). Antibiotic prophylaxis (p= 0.002) and malnutrition (p= 0.012) were linked with predicted decreased activity of metabolic pathways responsible for short chain fatty acid processing.ConclusionsIn infants with CF, gut microbiome composition and diversity differed between the two continents. Gut microbial diversity was not linked to growth. The relationship between malnutrition and antibiotic prophylaxis with reduced SCFA fermentation could have implications for gut health and function and warrants additional investigation.