Attenuation of leukocyte-endothelium interaction by antioxidant enzymes
Attenuation of leukocyte-endothelium interaction by antioxidant enzymes
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DOI：
10.1016/s0891-5849(03)00277-6
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发表时间：
2003-08-01
影响因子：
7.4
通讯作者：
Guo, ZM
中科院分区：
文献类型：
--
作者：
Yang, H;Shi, MJ;Guo, ZM
本研究探讨了铜锌超氧化物歧化酶和/或过氧化氢酶过表达对单个核细胞(MNC)和内皮细胞(ECs)相互作用的影响。取自野生型小鼠和高表达铜、锌超氧化物歧化酶和/或过氧化氢酶的转基因小鼠的主动脉内皮细胞。单核细胞取自野生型小鼠。经CuSO4氧化低密度脂蛋白(OxLDL)处理的野生型内皮细胞可显著上调血管细胞黏附分子-1(VCAM-1)和细胞间黏附分子-1(ICAM-1)的表达，增加MNC的黏附能力。在内皮细胞中过表达铜、锌超氧化物歧化酶和/或过氧化氢酶可减弱oxLDL诱导的MNC黏附和细胞黏附分子的表达。例如，高表达铜、锌超氧化物歧化酶和/或过氧化氢酶的内皮细胞VCAM-1和ICAM-1的表达显著低于野生型内皮细胞，贴壁的单核细胞数量也明显少于野生型内皮细胞。此外，联合高表达铜、锌超氧化物歧化酶和过氧化氢酶的内皮细胞表达VCAM-1和ICAM-1的能力明显低于单独高表达铜、锌超氧化物歧化酶和过氧化氢酶的内皮细胞，黏附的单核细胞数也明显减少。这些结果提示，联合过表达铜、锌超氧化物歧化酶和过氧化氢酶比单独过表达铜、锌超氧化物歧化酶或过氧化氢酶能更有效地减少细胞黏附分子的表达，抑制白细胞与内皮细胞的黏附。(C)2003年爱思唯尔公司。
This report assessed the effect of overexpressing Cu,Zn superoxide dismutase (SOD) and/or catalase on the interaction of mononuclear cells (MNCs) and endothelial cells (ECs). ECs were obtained from the aorta of wild-type mice and transgenic mice overexpressing Cu,ZnSOD and/or catalase. MNCs were obtained from wild-type mice. Treatment of wild-type ECs with CuSO4-oxidized low-density lipoprotein (oxLDL) significantly elevated the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) and increased the adherence of MNCs. Overexpression of Cu,ZnSOD and/or catalase in ECs attenuated the adherence of MNCs and the expression of cell adhesion molecules induced by oxLDL. For example, ECs overexpressing Cu,ZnSOD and/or catalase showed significantly less expression of VCAM-1 and ICAM-1 and less number of adherent MNCs than wild-type ECs. Moreover, ECs overexpressing Cu,ZnSOD and catalase in combination showed significantly less expression of VCAM-1 and ICAM-1 and less number of adherent MNCs than those overexpressing either Cu,ZnSOD or catalase alone. These results suggest that combinational overexpression of Cu,ZnSOD and catalase can reduce the expression of cell adhesion molecules and inhibit the adherence of leukocyte to ECs more efficiently than overexpression of Cu,ZnSOD or catalase alone. (C) 2003 Elsevier Inc.