Genome size characterization of the principal endocellular symbiotic bacteria of the weevil Sitophilus oryzae, using pulsed field gel electrophoresis
Genome size characterization of the principal endocellular symbiotic bacteria of the weevil Sitophilus oryzae, using pulsed field gel electrophoresis
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DOI：
10.1016/s0965-1748(97)00005-2
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发表时间：
1997-04-01
影响因子：
3.8
通讯作者：
Nardon, P
中科院分区：
文献类型：
--
作者：
Charles, H;Condemine, G;Nardon, P
采用脉冲保持凝胶电泳（PFGE）技术测定了米象象（Sitophilus oryzae）主要胞内共生细菌（内吞菌）的染色体大小。限制性内切酶SpeI、SwaI和XbaI分别产生22、23和24个片段。片段大小从15到460 kb不等，共生细菌的基因组大小估计约为3.0 Mb。当运行全部未消化的DNA时，发现了一个额外的片段，应该对应于138 kb的质粒。大肠杆菌的基因组大小（4.7 Mb）与共生细菌的基因组大小（3.0 Mb）的差异可能与宿主内胞生物的细胞内进化有关。在I-CeuI酶切中发现的rDNA （23S）位点不超过2个，这说明在进化过程中不存在序列片段基因缺失。1997爱思唯尔科学有限公司
The chromosome size of the principal intracellular symbiotic bacteria (endocytobiotes) of the weevil Sitophilus oryzae has been determined by pulsed held gel electrophoresis (PFGE). The restriction enzymes SpeI, SwaI and XbaI generated 22, 23 and 24 fragments, respectively. The fragment sizes ranged from 15 to 460 kb and the genome size of the symbiotic bacteria was estimated to be approximately 3.0 Mb. An additional fragment was seen when running total undigested DNA and should correspond to a plasmid of 138 kb. The principal symbiotic bacteria belong to the enterobacteriaceae family and are closely related to Escherichia coli, The difference between the genome size of E., coli (4.7 Mb) and symbiotic bacteria (3.0 Mb) was discussed and might be related to the intracellular evolution of the endocytobiotes within their host, No more than two rDNA (23S) loci were found with I-CeuI digestion, which argues against a serial fragment gene deletion in the course of evolution. (C) 1997 Elsevier Science Ltd.