High efficiency, long-term clinical expression of cottontail rabbit papillomavirus (CRPV) DNA in rabbit skin following particle-mediated DNA transfer.
High efficiency, long-term clinical expression of cottontail rabbit papillomavirus (CRPV) DNA in rabbit skin following particle-mediated DNA transfer.
复制标题
颗粒介导的 DNA 转移后，棉尾兔乳头瘤病毒 (CRPV) DNA 在兔皮肤中高效、长期临床表达。
DOI：
10.1093/nar/24.13.2620
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发表时间：
1996
影响因子：
14.9
通讯作者：
Brandsma,JL
中科院分区：
文献类型：
--
作者：
Xiao,W;Brandsma,JL
在接种棉尾兔乳头瘤病毒（CRPV）DNA的兔中评价皮肤支持用颗粒介导系统递送的基因的持久表达的能力。在瞬时β-半乳糖苷酶测定中确定在兔皮肤中最大基因表达的最佳递送力。然后以350-400 p.s.i.接种4只家兔的45个部位。CRPV DNA。所有部位（100%）均形成乳头状瘤，多数部位为多发性乳头状瘤。这些结果支持使用颗粒介导的递送系统进行基因治疗的可行性，并表明某些乳头瘤病毒特征，例如复制起点，可能非常适合用于载体中以靶向皮肤的长期表达。
The ability of skin to support long lasting expression of genes delivered with a particle-mediated system was evaluated in rabbits inoculated with cottontail rabbit papillomavirus (CRPV) DNA. The optimal delivery force for maximal gene expression in rabbit skin was determined in transient β-galactosidase assays. Forty-five sites in four rabbits were then inoculated at 350–400 p.s.i. with CRPV DNA. All sites (100%) formed papillomas with multiple papillomas at most sites. These results support the feasibility of using a particle-mediated delivery system for gene therapy and suggest that some papillomavirus features, such an origin of replication, may be well suited for use in vectors to target long term expression to skin.