Comparative analysis of expressed genes from cacao meristems infected by Moniliophthora perniciosa
Comparative analysis of expressed genes from cacao meristems infected by Moniliophthora perniciosa
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DOI：
10.1093/aob/mcm092
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发表时间：
2007-07-01
期刊：
影响因子：
4.2
通讯作者：
Cascardo, Júlio C. M.
中科院分区：
文献类型：
--
作者：
Gesteira, Abelmon S.;Micheli, Fabienne;Cascardo, Júlio C. M.
背景与目的女巫扫帚病是由半生养型担子菌Moniliophthora perniciosa引起的可可树病害，是西半球可可树上最重要的病害之一。因为很少是知道的全球过程中，这种疾病的发展，表达序列标签（EST）被用来确定在可可-Moniliophthora perniciosainteraction.Methods两个cDNA文库中表达的基因，对应的抗性（RT）和敏感（SP）可可-M。使用DB SMART Creator cDNA文库试剂盒（Clontech）从总RNA构建有害相互作用。随机选择克隆，从5'端测序，并使用生物信息学工具进行分析，包括差异基因表达的计算机分析。Key Results从RT和SP cDNA文库中共产生6884个EST。这些ESTs由2585个单联体和341个重叠群组成，共2926个非冗余序列。与已经发表的其他几个可可图书馆相比，这些图书馆的冗余度低，特异性高。序列分析允许为54%的序列分配一个假定的功能类别，而大约。22%的序列对应于未知的功能，大约20%的序列对应于未知的功能。24%的序列与数据库中存在的其他蛋白质没有显示出任何显著的相似性。尽管两个文库之间的功能类别中序列的总体分布相似，但观察到了定性差异。鉴定了参与病原菌感染防御反应或程序性细胞死亡的基因，如致病相关蛋白、胰蛋白酶抑制剂或草酸氧化酶，其中一些基因在抗病和感病互作中表现出差异表达。perniciosa相互作用，相信这将为理解可可对M. perniciosa，制定战略，以控制丛枝病，并作为一个来源的多态性的分子标记开发和标记辅助选择。
Background and Aims Witches' broom disease is caused by the hemibiotrophic basidiomycete Moniliophthora perniciosa, and is one of the most important diseases of cacao in the western hemisphere. Because very little is known about the global process of such disease development, expressed sequence tags (ESTs) were used to identify genes expressed during the Theobroma cacao -Moniliophthora perniciosa interaction.Methods Two cDNA libraries corresponding to the resistant (RT) and susceptible (SP) cacao-M. perniciosa interactions were constructed from total RNA, using the DB SMART Creator cDNA library kit (Clontech). Clones were randomly selected, sequenced from the 5' end and analysed using bioinformatics tools including in silico analysis of the differential gene expression.Key Results A total of 6884 ESTs were generated from the RT and SP cDNA libraries. These ESTs were composed of 2585 singlets and 341 contigs for a total of 2926 non-redundant sequences. The redundancy of the libraries was low and their specificity high when compared with the few other cacao libraries already published. Sequence analysis allowed the assignment of a putative functional category for 54 % of sequences, whereas approx. 22 % of sequences corresponded to unknown function and approx. 24 % of sequences did not show any significant similarity with other proteins present in the database. Despite the similar overall distribution of the sequences in functional categories between the two libraries, qualitative differences were observed. Genes involved during the defence response to pathogen infection or in programmed cell death were identified, such as pathogenesis related-proteins, trypsin inhibitor or oxalate oxidase, and some of them showed an in silico differential expression between the resistant and the susceptible interactions.Conclusions As far as is known this is the first EST resource from the cacao-M. perniciosa interaction and it is believed that it will provide a significant contribution to the understanding of the molecular mechanisms of the resistance and susceptibility of cacao to M. perniciosa, to develop strategies to control witches broom, and as a source of polymorphism for molecular marker development and marker-assisted selection.