Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains
Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains
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DOI：
10.1016/j.fsi.2018.11.017
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发表时间：
2019-01-01
影响因子：
4.7
通讯作者：
Liu, Chun-Hung
中科院分区：
文献类型：
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作者：
Wang, Yu-Chu;Hu, Shao-Yang;Liu, Chun-Hung
含有戊糖乳杆菌BD6、发酵乳杆菌LW2、枯草芽孢杆菌E20和酿酒酵母P13的复合益生菌对虾生长和健康状况改善的益生功效优于使用单一益生菌。10⁸菌落形成单位（cfu）/（千克饲料）水平的益生菌混合物以及含有10⁹ cfu/（千克饲料）的BD6和E20的饲料显著改善了虾的生长和健康状况，而含有P13或LW2的饲料对虾的生长没有显著影响。对照组和各处理组之间的虾体成分没有显著差异。投喂56天后，投喂含有益生菌混合物（10⁷ - 10⁹ cfu/（千克饲料））饲料的虾在注射溶藻弧菌后的存活率更高，但需要投喂10⁹ cfu/（千克饲料）的单一益生菌（酿酒酵母P13除外）才能提高虾的存活率。投喂益生菌混合物的虾组具有更好的抗病性可能是由于血细胞的酚氧化酶活性、呼吸爆发和溶菌酶活性增加。因此，我们认为该益生菌混合物能够为南美白对虾提供足够的益生功效，推荐使用含有10⁸ cfu/（千克饲料）益生菌混合物的饲料。
The probiotic efficiencies of the mixed probiotics containing Lactobacillus pentosus BD6, Lac. fermentwn LW2, Bacillus subtilis E20, and Saccharomyces cerevisiae P13 for shrimp growth and health status improvement were better than those when using single probiotics. The probiotic mixture at a level of 10(8) colony-forming units (cfu) (kg diet)(-1) and the diets containing BD6 and E20 at 10(9) cfu (kg diet)(-1) significantly improved the growth and health status of shrimp, whereas the diets containing P13 or LW2 did not significantly affect the growth of shrimp. No significant difference in the carcass composition was recorded among the control and treatments. After 56 days of feeding, shrimp fed the diet containing the probiotic mixture (10(7)similar to 10(9) cfu (kg diet)(-1)) had higher survival after injection with the V. alginolyticus, but 10(9) cfu (kg diet)(-1) of single probiotics (except for S. cerevisiae P13) had to be administered to improve shrimp survival. The better disease resistance of shrimp in groups fed the probiotic mixture might have been due to increased phenoloxidase activity, respiratory bursts, and lysozyme activity of hemocytes. Therefore, we considered that the probiotic mixture could adequately provide probiotic efficiency for white shrimp, and a diet containing 10(8) cfu (kg diet)(-1) probiotic mixture is recommended.