A standardized definition of placental infection by SARS-CoV-2, a consensus statement from the National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop.
A standardized definition of placental infection by SARS-CoV-2, a consensus statement from the National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop.
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DOI：
10.1016/j.ajog.2021.07.029
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发表时间：
2021-12
影响因子：
9.8
通讯作者：
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop
中科院分区：
文献类型：
--
作者：
Roberts DJ;Edlow AG;Romero RJ;Coyne CB;Ting DT;Hornick JL;Zaki SR;Das Adhikari U;Serghides L;Gaw SL;Metz TD;National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop
感染SARS-CoV-2的妊娠个体比非妊娠个体有更高的重症监护室入院率、氧气需求、机械通气需求和死亡率。COVID-19疾病严重程度的增加可能与病毒血症和胎盘感染的风险增加有关。母体SARS-CoV-2感染也与妊娠并发症有关，如先兆子痫和早产，这些并发症可以通过胎盘介导或反映在胎盘中。母体病毒血症后发生胎盘感染可能导致母婴传播（垂直传播），影响1%至3%的新生儿。然而，胎盘感染没有公认的或标准的定义。美国国立卫生研究院/尤妮斯·肯尼迪·施莱佛国家儿童健康和人类发展研究所召集了一个专家组，提出了胎盘感染的工作定义，为正在进行的妊娠期间SARS-CoV-2研究提供信息。专家建议，胎盘感染应使用以下一种或多种方法在胎盘组织中进行病毒检测和定位的技术进行定义：使用反义探针（检测复制）或正义探针（检测病毒信使RNA）进行原位杂交，或使用免疫组织化学检测病毒核衣壳或刺突蛋白。如果上述方法是不可能的，逆转录聚合酶链反应检测或定量胎盘匀浆中的病毒RNA，或电子显微镜是替代方法。提出了胎盘感染可能性的分级分类，即确定、很可能、可能和不太可能。报告胎盘感染的患者应描述采样方法（采集样本的位置和数量）、组织保存方法和检测技术。对胎盘处理、检查和采样以及使用经验证的试剂和样本方案提出了建议（作为附录纳入）。
Pregnant individuals infected with SARS-CoV-2 have higher rates of intensive care unit admission, oxygen requirement, need for mechanical ventilation, and death than nonpregnant individuals. Increased COVID-19 disease severity may be associated with an increased risk of viremia and placental infection. Maternal SARS-CoV-2 infection is also associated with pregnancy complications such as preeclampsia and preterm birth, which can be either placentally mediated or reflected in the placenta. Maternal viremia followed by placental infection may lead to maternal-fetal transmission (vertical), which affects 1% to 3% of exposed newborns. However, there is no agreed-upon or standard definition of placental infection. The National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development convened a group of experts to propose a working definition of placental infection to inform ongoing studies of SARS-CoV-2 during pregnancy. Experts recommended that placental infection be defined using techniques that allow virus detection and localization in placental tissue by one or more of the following methods: in situ hybridization with antisense probe (detects replication) or a sense probe (detects viral messenger RNA) or immunohistochemistry to detect viral nucleocapsid or spike proteins. If the abovementioned methods are not possible, reverse transcription polymerase chain reaction detection or quantification of viral RNA in placental homogenates, or electron microscopy are alternative approaches. A graded classification for the likelihood of placental infection as definitive, probable, possible, and unlikely was proposed. Manuscripts reporting placental infection should describe the sampling method (location and number of samples collected), method of preservation of tissue, and detection technique. Recommendations were made for the handling of the placenta, examination, and sampling and the use of validated reagents and sample protocols (included as appendices).
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影响因子：
120.7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.15585/mmwr.mm6938e1
发表时间：
2020-09-25
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
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作者：
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通讯作者：
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DOI：
10.1093/cid/ciab623
发表时间：
2022-03-23
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
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作者：
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通讯作者：
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影响因子：
3.3
作者：
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