ELEVATED INSULIN-RECEPTOR CONTENT IN HUMAN BREAST-CANCER
ELEVATED INSULIN-RECEPTOR CONTENT IN HUMAN BREAST-CANCER
复制标题
DOI：
10.1172/jci114868
复制
发表时间：
1990-11-01
影响因子：
15.9
通讯作者：
VIGNERI, R
中科院分区：
文献类型：
--
作者：
PAPA, V;PEZZINO, V;VIGNERI, R
乳腺癌细胞的生长受激素、生长因子及其受体的调节。在本研究中，我们采用了一种新的，敏感的，和具体的放射免疫法直接测量的胰岛素受体在乳腺癌手术标本。在159例标本中，胰岛素受体含量为6.15 ±。3.69 ng/0.1 mg蛋白质。在全乳切除术后获得的27例正常乳腺组织（0.95 ± 0.68，P < 0.001）和6例乳房缩小术后获得的正常乳腺组织（0.84 ± 0.78，P < 0.001）中，该值均比平均值高6倍以上。乳腺癌组织中的胰岛素受体含量也高于所研究的任何正常组织（包括肝脏）（Pezzino，V.，V. Papa，V. Trischitta，A. Brunetti，P.A.古德曼，M.K. Treutelaar，J.A.威廉姆斯，文学士马杜克斯河维芙奈里和身份证戈德费恩1989. Am. 257：E451-457）。乳腺癌中的胰岛素受体保留了其结合胰岛素和经历胰岛素诱导的酪氨酸激酶活化的能力。免疫组化结果显示，胰岛素受体存在于恶性上皮细胞，但在间质和炎性细胞中未检测到。单因素分析显示胰岛素受体含量与肿瘤大小（P = 0.014）、组织学分级（P = 0.030）和雌激素受体含量（P = 0.035）呈正相关。胰岛素受体含量与患者的年龄、体重、绝经状态和淋巴结受累之间无显著相关性。因此，这些研究表明，乳腺癌中胰岛素受体含量增加，并提高了胰岛素受体可能在这些肿瘤的生物学中发挥作用的可能性。
The growth of breast cancer cells is under the regulation of hormones, growth factors, and their receptors. In the present study, we have employed a new, sensitive, and specific radioimmunoassay for the direct measurement of insulin receptors in surgical specimens of breast cancers. In 159 specimens the insulin receptor content was 6.15 .+-. 3.69 ng/0.1 mg protein. This value was more than sixfold higher than the mean value in both 27 normal breast tissues obtained at total mastectomy (0.95 + 0.68, P < 0.001) and in six normal specimens obtained from reduction mammoplasty (0.84 + 0.78, P < 0.001). The insulin receptor content in breast cancer tissues was also higher than in any normal tissue investigated including liver (Pezzino, V., V. Papa, V. Trischitta, A. Brunetti, P.A. Goodman, M.K. Treutelaar, J.A. Williams, B.A. Maddux R. Vivneri, and I.D. Goldfine. 1989. Am. J. Physiol. 257:E451-457). The insulin receptor in breast cancer retained its ability to both bind insulin and undergo insulin-induced tyrosine kinase activation. Immunostaining of the specimens revealed that the insulin receptor was present in malignant epithelial cells, but was not detected in stromal and inflammatory cells. Univariant analysis revealed that the insulin receptor content of the tumors correlated positively with tumor size (P = 0.014), histological grading (P = 0.030), and the estrogen receptor content (P = 0.035). There were no significant correlations between insulin receptor content and the age, body weight, menopausal status, and nodal involvement of the patients. These studies indicate, therefore, that the insulin receptor content is increased in breast cancers and raise the possibility that the insulin receptor may have a role in the biology of these tumors.