Effects of hypertonic dextrose injections in the rabbit carpal tunnel.
Effects of hypertonic dextrose injections in the rabbit carpal tunnel.
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高渗葡萄糖注射对兔腕管的影响。
DOI：
10.1002/jor.21297
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Amadio,PeterC
中科院分区：
文献类型：
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作者：
Yoshii,Yuichi;Zhao,Chunfeng;Schmelzer,JamesD;Low,PhillipA;An,Kai-Nan;Amadio,PeterC
目的研究不同剂量高渗葡萄糖注射对兔腕管滑膜下结缔组织（SSCT）和正中神经的影响。方法选用体重4.0 ~ 4.5 kg的新西兰大白兔38只。一只前爪腕管随机注射五种不同治疗方法中的一种：生理盐水-单次注射；生理盐水-间隔1周注射两次；10%葡萄糖-单次注射；20%葡萄糖-单次注射；或10%葡萄糖-间隔1周注射两次。初始注射后12周处死动物，进行电生理（EP）、SSCT力学测试和组织学评估。结果与生理盐水注射组相比，10%葡萄糖双注射组SSCT的能量吸收显著增加。与其他组相比，10%葡萄糖双注射组的SSCT僵硬度也显著增加。与生理盐水单次注射组相比，10%葡萄糖双注射组的SSCT厚度显著增加，10%葡萄糖双注射组的神经短长直径比显著降低。各组之间的EP没有变化。结论：葡萄糖注射后sssct纤维化存在长达12周；多次注射会产生更大的影响，包括神经变平的继发性变化。该模型可用于研究外部纤维化对神经形态学和生理学的影响，如临床发生在腕管综合征。©2011 Orthopaedic Research Society by Wiley journals, Inc。[J]中华骨科杂志，2011,31 (2):522 - 527
ObjectiveThis study investigated the effects of different doses of hypertonic dextrose injection on the carpal tunnel subsynovial connective tissue (SSCT) and median nerve in a rabbit model.MethodsThirty‐eight New Zealand white rabbits weighing 4.0–4.5 kg were used. One forepaw carpal tunnel was randomly injected with one of five different treatments: saline‐single injection; saline‐two injections 1 week apart; 10% dextrose‐single injection; 20% dextrose‐single injection; or 10% dextrose‐two injections 1 week apart. Animals were sacrificed at 12 weeks after the initial injection and were evaluated by electrophysiology (EP), SSCT mechanical testing and histology.ResultsThere were significant increases in the energy absorption of the SSCT in the 10% dextrose‐double injection group compared to the saline injection groups. SSCT stiffness was also significantly increased in the 10% dextrose‐double injection group compared to the other groups. There was a significant increase in the thickness of the SSCT in the 10% dextrose‐double injection group compared to the saline‐single injection group and a significant decrease in the nerve short‐long diameter ratio in the 10% dextrose‐double injection group compared to the saline‐single injection group. There were no changes in EP among the groups.ConclusionsSSCT fibrosis is present for up to 12 weeks after dextrose injection; multiple injections have bigger effects, including what appears to be a secondary change in nerve flattening. This model may be useful to study the effects of external fibrosis on nerve morphology and physiology, such as occurs clinically in carpal tunnel syndrome. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1022–1027, 2011