Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)
Definition of the tumor protein D52 (TPD52) gene family through cloning of D52 homologues in human (hD53) and mouse (mD52)
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DOI：
10.1006/geno.1996.0393
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发表时间：
1996-08-01
期刊：
影响因子：
4.4
通讯作者：
Basset, P
中科院分区：
文献类型：
--
作者：
Byrne, JA;Mattei, MG;Basset, P
报道了乳腺癌相关基因D52的同源基因的克隆，命名为D53，以及小鼠052基因的同源基因(HGMW批准的代码TPD52L1和TPD52)。人的D53蛋白和小鼠的D52蛋白分别与人的D52蛋白有52%和86%的相似性。对这三个蛋白质序列的分析发现，每个序列中都有一个螺旋卷曲结构域和N-和C末端的PEST结构域，D52和D53序列之间的同源性保持不变，再加上这些蛋白与已知蛋白质之间缺乏同源性，定义了一个新的哺乳动物基因/蛋白家族，即D52家族。人类D52基因座已被定位到染色体8q21，本研究利用原位定位技术，将人类D53基因座定位到染色体6q22-q23。我们观察到人类052和D53基因在一些乳腺肿瘤及其衍生细胞系中共表达，发现雌激素受体阳性的MCF7乳腺癌细胞中D52和D53基因转录水平的维持依赖于雌二醇。然而，D52和D53基因分别在HL-60和K-562白血病细胞中特异表达，12-O-十四烷基佛波醇-13-乙酸酯处理后，这些细胞系中D52和D53的转录水平降低。卷曲结构域的存在，以及观察到的D52和D53基因的共同或独立表达，表明D52和D53蛋白可能能够形成异源和/或同源二聚体。(C)1996年学术出版社。
Cloning is reported of a cDNA homologue to the breast carcinoma-associated D52 cDNA, termed D53, and of a mouse 052 cDNA (HGMW-approved symbols TPD52L1 and TPD52). Human D53 and mouse D52 proteins are predicted to be 52 and 86% identical to human D52, respectively. Analysis of the three protein se quences identified a coiled-coil domain and N- and C terminally located PEST domains in each, The conservation of homology between the D52 and the D53 sequences, combined with a lack of homology between these and known proteins, defines a new mammalian gene/protein family, the D52 family. The human D52 locus has been previously mapped to chromosome 8q21, and using in situ mapping in the present study, a human D53 locus was mapped to chromosome 6q22-q23. We observed coexpression of the human 052 and D53 genes in some breast tumors and derivative cell lines and found that maintenance of D52 and D53 transcript levels in estrogen receptor-positive MCF7 breast carcinoma cells depends upon estradiol. However, D52 and D53 genes were specifically expressed in HL-60 and K-562 leukemia cells, respectively, with 12-O-tetradecanoylphorbol-13-acetate treatment decreasing D52 and D53 transcript levels in these cell lines. The presence of a coiled-coil domain, combined with observed co- or independent expression of the D52 and D53 genes, suggests that D52 and D53 proteins may be capable of hetero and/or homodimer formation. (C) 1996 Academic Press, Inc.