In Vitro and In Silico Antioxidant Activity of Novel Peptides Prepared from Paeonia Ostii 'Feng Dan' Hydrolysate
In Vitro and In Silico Antioxidant Activity of Novel Peptides Prepared from Paeonia Ostii 'Feng Dan' Hydrolysate
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芍药·冯丹·水解液制备的新型肽的体外和计算机抗氧化活性
DOI：
10.3390/antiox8100433
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发表时间：
2019-10-01
期刊：
影响因子：
7
通讯作者：
Shen, Yehua
中科院分区：
文献类型：
--
作者：
Wang, Min;Li, Cong;Shen, Yehua
天然产物抗氧化肽具有优越的性能和广阔的应用前景。本研究以芍药籽粕为原料制备了5种新型抗氧化肽，并利用量子化学方法对其生物活性及结构与性能之间的关系进行了研究。以自由基清除活性为指标，通过5种蛋白酶和分离技术纯化浓缩抗氧化肽。采用纳米液相色谱-串联质谱（LC-MS/MS）对FSAP （Phe-Ser-Ala-Pro）、PVETVR （pro - val - glu - glu - thr - val - arg）、QEPLLR （gln - glu - pro - leu - arg）、EAAY （Glu-Ala-Ala-Tyr）和VLRPPLS （Val-Leu-Arg-Pro-Pro-Leu-Ser）进行了鉴定。体外抗氧化活性试验表明，在0.5 mg/mL浓度下，EAAY对2,2′-氮化二苯醚（ABTS）和羟基自由基的清除率分别为98.5%±1.1%和61.9%±1.3% （p < 0.01）。利用密度泛函理论（DFT）以B3LYP/6-31G*基集进行了计算机计算。根据天然键轨道（NBO）分析，推测其具有清除自由基的生物活性。抗氧化肽对L929成纤维细胞无明显的细胞毒性。因此，该种子副产物的多肽在功能性食品、保健品和药理学产品等方面具有良好的应用前景。
Antioxidant peptides derived from natural products have superior performance and broader application prospects. In this study, five novel antioxidant peptides were prepared from Paeonia ostii (P. ostii) seed meal, moreover the bioactive and the relationship between structure and properties of antioxidant peptides were elucidated by quantum chemical calculations. The free radical-scavenging activities were used as indexes to purify and concentrate the antioxidant peptides through five proteases and separation techniques. FSAP (Phe-Ser-Ala-Pro), PVETVR (Pro-Val-Glu-Thr-Val-Arg), QEPLLR (Gln-Glu-Pro-Leu-Leu-Arg), EAAY (Glu-Ala-Ala-Tyr) and VLRPPLS (Val-Leu-Arg-Pro-Pro-Leu-Ser) were identified by nano liquid chromatography–tandem mass spectrometry (LC-MS/MS). In vitro antioxidant activity test, EAAY exhibited the highest 2, 2’-azino-bis (ABTS) and hydroxyl radical-scavenging activity of 98.5% ± 1.1% and 61.9% ± 1.3%, respectively (p < 0.01), at 0.5 mg/mL. In silico calculations were carried out using the density functional theory (DFT) with the B3LYP/6-31G* basis set. According to natural bond orbital (NBO) analysis, the bioactivity of free-radical scavenging of the peptides was presumed. Moreover, the antioxidant peptides demonstrated no obvious cytotoxicity to L929 fibroblast cells. Therefore, the peptides from P. ostii seed by-products might potentially have excellent uses in functional foods, nutraceuticals and pharmacological products.