Podoplanin, ezrin, and Rho-A proteins may have joint participation in tumor invasion of lip cancer
Podoplanin, ezrin, and Rho-A proteins may have joint participation in tumor invasion of lip cancer
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DOI：
10.1007/s00784-016-1956-3
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发表时间：
2017-06-01
影响因子：
3.4
通讯作者：
Oliveira, Denise Tostes
中科院分区：
文献类型：
--
作者：
Assao, Agnes;Nonogaki, Suely;Oliveira, Denise Tostes
Podoplanin和Ezrin通过Rho-A磷酸化相互作用，参与了口腔鳞状细胞癌的侵袭和细胞运动过程。本研究旨在探讨Podoplanin、Ezrin和Rho-A在91例下唇鳞状细胞癌中的表达及其对患者预后的影响。和Rho-A通过半定量评分法进行评价，基于在肿瘤侵袭前沿捕获的10个显微镜视野。通过Fischer精确检验和斯皮尔曼检验证实这些蛋白与临床病理特征的关联和相关性。用Kaplan-Meier法和logrank检验分析其预后价值。（p = 0.024），局部复发（p = 0.028），和淋巴结转移（pN+）podoplanin在胞膜（p = 0.000和r = 0.384）和胞浆（p = 0.000和r = 0.344）的表达与ezrin的表达有统计学相关性。此外，膜podoplanin与Rho-A表达显著相关（p = 0.006和r = 0.282）。podoplanin、ezrin和Rho-A的表达不是下唇鳞状细胞癌患者的显著预后因素。因此，我们的研究结果证实了podoplanin、ezrin和Rho-A在唇鳞状细胞癌中的表达之间的相关性，表明这些蛋白协同参与细胞运动和侵袭。此外，胞浆podoplanin强表达可能有助于识别唇鳞状细胞癌患者，并降低局部区域复发的风险。
Podoplanin and ezrin connection through Rho-A phosphorylation have been suggested as part of the activation pathway, in the process of tumor invasion and cell movement in oral squamous cell carcinomas.The aim of this study was to evaluate the correlation among podoplanin, ezrin, and Rho-A immunoexpressions in 91 squamous cells carcinomas of the lower lip and their influence in patient's prognosis.The immunoexpressions of podoplanin, ezrin, and Rho-A were evaluated through a semi-quantitative score method, based on the capture of 10 microscopic fields at the front of tumor invasion. The association and correlation of these proteins with the clinicopathological features were verified by Fischer's exact test and Spearman's test. The prognostic values were analyzed by Kaplan-Meier method and log-rank test.A statistically significant association between strong cytoplasmic podoplanin expression and alcohol (p = 0.024), loco-regional recurrences (p = 0.028), and lymph node metastasis (pN+) (p = 0.010) was found. The membranous (p = 0.000 and r = 0.384) and cytoplasmic (p = 0.000 and r = 0.344) podoplanin expression was statistically correlated with ezrin expression. Also, membranous podoplanin was significantly correlated with Rho-A expression (p = 0.006 and r = 0.282). The expressions of podoplanin, ezrin, and Rho-A were not significant prognostic factors for patients with squamous cell carcinomas of the lower lip.Therefore, our results confirm a correlation among podoplanin, ezrin, and Rho-A expressions in squamous cell carcinoma of the lip suggesting a cooperative participation of these proteins in cell movement and invasion.Furthermore, strong cytoplasmic podoplanin expression could be helpful to identify patients with squamous cell carcinoma of the lip and lower risk of loco-regional recurrences.