CD34 counts to predict the adequate collection of peripheral blood progenitor cells
CD34 counts to predict the adequate collection of peripheral blood progenitor cells
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DOI：
10.1038/sj.bmt.1700938
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发表时间：
1997-10-01
影响因子：
4.8
通讯作者：
Navarrete, C
中科院分区：
文献类型：
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作者：
Armitage, S;Hargreaves, R;Navarrete, C
成功获取足够的自体外周血祖细胞(PBPC)用于植入的一个基本前提是最佳的采集时间。分析了一些外周血祖细胞的替代标志物，以确定可以预测最佳采集时间的单一测试，提供至少2x10(6)CD34(+)细胞/公斤患者体重。这项研究包括95名接受不同动员方案的患者，他们同时接受了化疗和G-CSF治疗实体肿瘤和血液系统恶性肿瘤。仅收集到的PBPC就有157个。对动员期间和采集前(采集前)采集的血样进行全血细胞计数(FBC)和CD34(+)细胞计数。除FBC外，还对所有PBPC收集的集落形成细胞和CD34(+)细胞进行了分析。采集当天的白细胞计数与采集的CD34(+)细胞总数呈弱相关(r=0.30)。相反，循环中CD34(+)细胞的绝对数量与CD34(+)细胞和相应采集物的CFU-GM产量密切相关。如果动员样本含有大于或等于20×10(6)CD34(+)细胞/毫升，第二天进行的单次采集中94%含有大于或等于2×10(6)CD34(+)细胞/千克。
An essential prerequisite for successful procurement of sufficient autologous peripheral blood progenitor cells (PBPC) for engraftment is the optimal timing of collection. A number of surrogate markers of peripheral blood progenitor cells were analysed to identify a single test which could predict the optimum time to harvest, providing at least 2 x 10(6) CD34(+) cells/kg patient body weight. The study comprised 95 patients undergoing varied mobilisation regimens with chemotherapy and G-CSF for both solid tumours and haematological malignancies. One hundred and fifty-seven PBPC harvests mere collected. Full blood counts (FBC) and CD34(+) cell enumeration was performed on blood samples taken during the mobilisation period and immediately prior to leucapheresis (pre-harvest). All PBPC collections were assayed for colony-forming cells and CD34(+) cells in addition to a FBC. The white cell count on the day of harvest showed only weak correlation with the total number of CD34(+) cells in the collection (r = 0.30). In contrast, the absolute number of circulating CD34(+) cells strongly correlated with the CD34(+) cell and CFU-GM yield of the corresponding apheresis product. Provided the mobilisation sample contained greater than or equal to 20 x 10(6) CD34(+) cells/ml, 94% of single collections, performed the following day, contained greater than or equal to 2 x 10(6) CD34(+) cells/kg.