Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung
Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung
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DOI：
10.1158/1078-0432.ccr-05-2566
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发表时间：
2006-04-15
影响因子：
11.5
通讯作者：
Mukai, K
中科院分区：
文献类型：
--
作者：
Semba, S;Iwaya, K;Mukai, K
目的：高度侵袭性和转移性癌细胞，如肺腺癌细胞，通过组装肌动蛋白丝的分支网络形成不规则突起。在哺乳动物细胞中，肌动蛋白相关蛋白2和3（Arp 2/3）复合物通过与Wiskott-Aldrich综合征（WASP）/WASP家族维脯氨酸同源蛋白2（WAVE 2）结合而启动肌动蛋白组装以形成板状伪足突起。在这项研究中，共定位的Arp 2和WAVE 2在肺腺癌进行了调查，以阐明其预后value.Experimental设计：免疫组化染色的Arp 2和WAVE 2做了115肺腺癌的病理阶段IA到IIIA类镜像部分。Kaplan-Meier无病生存曲线和总生存曲线分析Arp 2和WAVE 2共表达的预后意义。结果：在115例肺癌标本中，78例（67.8%）同一肿瘤细胞同时表达Arp 2和WAVE 2。Arp 2和WAVE 2同时表达的癌细胞比例在有淋巴结转移的病例中显著增高（P=0.0046），而在细支气管肺泡癌中显著降低（P
Purpose: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling a branched network of actin filaments. in mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous protein 2 (WAVE2). In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate its prognostic value.Experimental Design: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic stage IA to IIIA classes. Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic significance of the coexpression of Arp2 and WAVE2.Results: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens. The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis (P=0.0046), and significantly lower in bronchioloalveolar carcinomas (P